Antiviral Resistance Protein Tm-2(2) Functions on the Plasma Membrane

文献类型： 外文期刊

第一作者： Chen, Tianyuan

作者： Chen, Tianyuan;Liu, Dan;Niu, Xiaolin;Wang, Junzhu;Qian, Lichao;Han, Lu;Liu, Na;Zhao, Jinping;Liu, Yule;Zhao, Jinping;Hong, Yiguo

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期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

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收录情况： SCI

摘要： The tomato Tobacco mosaic virus resistance-2(2) (Tm-2(2)) gene encodes a coiled-coil-nucleotide binding site-Leu-rich repeat protein lacking a conventional plasma membrane (PM) localization motif. Tm-2(2) confers plant extreme resistance against tobamoviruses including Tobacco mosaic virus (TMV) by recognizing the avirulence (Avr) viral movement protein (MP). However, the subcellular compartment where Tm-2(2) functions is unclear. Here, we demonstrate that Tm-2(2) interacts with TMV MP to form a protein complex at the PM. We show that both inactive and active Tm-2(2) proteins are localized to the PM. When restricted to PM by fusing Tm-2(2) to the S-acylated PM association motif, the Tm-2(2) fusion protein can still induce a hypersensitive response cell death, consistent with its activation at the PM. Through analyses of viral MP mutants, we find that the plasmodesmata (PD) localization of the Avr protein MP is not required for Tm-2(2) function. These results suggest that Tm-2(2)-mediated resistance takes place on PM without requirement of its Avr protein to be located to PD.

分类号： Q945

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