Molecular characterisation and functional analysis of a cytochrome P450 gene in cotton

文献类型: 外文期刊

第一作者: Zhou, Kexue

作者: Zhou, Kexue;Long, Lu;Sun, Quan;Wang, Weina;Gao, Wei;Cai, Chaowei;Mo, Jianchuan;Cheng, Jieru;Zhang, Xiangrui;Liu, Yujia;Miao, Chen;Zhang, Xiao;Cai, Yingfan;Du, Xiongming;Shi, Yuzhen;Yuan, Youlu;Chu, Zongyan

作者机构:

关键词: cotton;cytochrome P450;VIGS;Verticillium wilt;function

期刊名称:BIOLOGIA ( 影响因子:1.35; 五年影响因子:1.139 )

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年卷期:

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收录情况: SCI

摘要: Verticillium wilt causes devastating loss of yield and quality in many crops, including cotton. To determine the molecular mechanism of resistance to verticillium wilt in cotton, we isolated a new cytochrome P450 gene, CYP94C1, and analysed its function. We obtained the complete open reading frame, which encodes a protein of 500 amino acids. The results of the functional analysis showed that resistance to verticillium wilt was enhanced when the gene was silenced using the virus-induced gene silencing (VIGS) method in cotton. GbCYP94C1-overexpressing Arabidopsis was more susceptible to Verticillium dahliae compared with the wild-type. In addition, GbCYP94C1 played a role in plant wound responses and in the expression of certain jasmonic acid (JA) signalling pathway. The results suggest that GbCYP94C1 plays a role in verticillium wilt resistance in cotton via the JA signalling pathway.

分类号: Q

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