Ultrasensitive detection of lead(II) using a turn-on probe based on the use of an aptamer and a water-soluble fluorescent perylene probe

文献类型： 外文期刊

第一作者： Yan, Mengmeng

作者： Yan, Mengmeng;Zhu, Chao;Huang, Yafei;Yan, Jiao;Chen, Ailiang;Yan, Mengmeng;Zhu, Chao;Huang, Yafei;Yan, Jiao;Chen, Ailiang;Huang, Yafei;Yan, Jiao

作者机构：

关键词： Heavy metal ions;ssDNA;Fluorescence;PTCDI;G-quadruplex

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The authors describe an ultra-sensitive approach for the determination of lead(II) by using a lead-specific aptamer and a fluorophore derived from perylenetetracarboxylic acid diimide (PTCDI). The fluorescence of PTCDI, with excitation/emission peaks at 494/543 nm, is quenched by the aptamer but restored if the aptamer binds lead(II). A detection scheme has been worked out that has a 0.1 ng center dot mL(-1)limit of detection and a dynamic range that extends from 0.1 ng center dot mL(-1) to 10 mu g center dot mL(-1). The assay also demonstrates high selectivity for lead ions over nine other ions due to the aptamer's high specificity. The method shows a average recovery of 77.2-93.4% with an RSD of less than 3% in water samples.

分类号： O65`O655

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