IL-22 suppresses the infection of porcine enteric coronaviruses and rotavirus by activating STAT3 signal pathway
文献类型: 外文期刊
第一作者: Xue, Mei
作者: Xue, Mei;Fu, Fang;Li, Lin;Ma, Yanlong;Shi, Hongyan;Zhang, Jiaoer;Feng, Li;Liu, Pinghuang;Zhao, Jing;Ying, Lan
作者机构:
关键词: Interleukin 22;Porcine rotavirus;Porcine epidemic diarrhea virus;Transmissible gastroenteritis virus;Intestinal epithelia;STAT3
期刊名称:ANTIVIRAL RESEARCH ( 影响因子:5.97; 五年影响因子:5.801 )
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收录情况: SCI
摘要: Interleukin-22 (IL-22), a member of the IL-10 superfamily, plays essential roles in fighting against mucosal microbial infection and maintaining mucosal barrier integrity within the intestine. However, little knowledge exists on the ability of porcine IL-22 (pIL-22) to fight against viral infection in the gut. In this study, we found that recombinant mature pIL-22 (mpIL-22) inhibited the infection of multiple diarrhea viruses, including alpha coronavirus, porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PoRV), in the intestinal porcine epithelial cell line J2 (IPEC-J2) cells. mpIL-22 up-regulated the expression of the antimicrobial peptide beta-defensin (BD-2), cytokine IL-18 and IFN-lambda. Furthermore, we found that mpIL-22 induced phosphorylation of STAT3 on Ser727 and Tyr705 in IPEC-J2 cells. Inhibition of STAT3 phosphorylation by S3I-201 abrogated the antiviral ability of mpIL-22 and the mpIL-22-induced expression of BD-2, IL-18, and IFN-lambda. Together, mpIL-22 inhibited the infection of PoRV and enteric coronaviruses, and up-regulated the expression of antimicrobial genes in IPEC-J2, which were mediated by the activation of the STAT3 signal pathway. The significant antiviral activity of IL-22 to curtail multiple enteric diarrhea viruses in vitro suggests that pIL-22 could be a novel therapeutic against devastating viral diarrhea in piglets. (C) 2017 Elsevier B.V. All rights reserved.
分类号: R37
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