Cloning of TaTPP-6AL1 associated with grain weight in bread wheat and development of functional marker

文献类型: 外文期刊

第一作者: Zhang, Pengfei

作者: Zhang, Pengfei;Wang, Chunping;Zhang, Pengfei;He, Zhonghu;Tian, Xiuling;Gao, Fengmei;Xu, Dengan;Liu, Jindong;Wen, Weie;Fu, Luping;Xia, Xianchun;Cao, Shuanghe;He, Zhonghu;Gao, Fengmei;Li, Genying;Sui, Xinxia

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关键词: CAPS marker;Linkage and association analysis;QTL;Thousand grain weight;TaTPP;Triticum aestivum

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

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收录情况: SCI

摘要: Trehalose 6-phosphate phosphatase (TPP) dephosphorylates trehalose 6-phosphate to trehalose, an important growth regulator, and is involved in starch accumulation and grain yield. In this study, wheat TPP homologs were isolated from chromosomes 6AL, 6BL, and 6DL, designated as TaTPP-6AL1, TaTPP-6BL1, and TaTPP-6DL1, respectively. Sequence alignment showed a single-nucleotide polymorphism (SNP) at TaTPP-6AL1 locus between cultivars with contrasting thousand grain weight (TGW), forming alleles TaTPP-6AL1a and TaTPP-6AL1b, respectively. A cleaved amplified polymorphic sequence (CAPS) marker, TaTPP-6AL1-CAPS, was developed to differentiate the two alleles. TaTPP-6AL1 was mapped within the interval of IWB65749 and IWB60449 in a recombinant inbred line (RIL) population derived from Zhou8425B/Chinese Spring using the wheat 90K SNP assay. A QTL for TGW identified in the interval explained 12.1-19.1% of the phenotypic variance across five environments. Association analysis on 141 Chinese wheat cultivars also indicated a significant correlation of TaTPP-6AL1 with TGW. In conclusion, TaTPP-6AL1 and its functional marker are valuable to improve grain yield in wheat breeding.

分类号: Q94

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