Isolation and characterization of a novel chalcone synthase gene family from mulberry

文献类型： 外文期刊

第一作者： Wang, Chuanhong

作者： Wang, Chuanhong;Zhi, Shuang;Liu, Changying;Xu, Fengxiang;Zhao, Aichun;Wang, Xiling;Tang, Xing;Yu, Maode;Wang, Chuanhong;Zhi, Shuang;Liu, Changying;Xu, Fengxiang;Zhao, Aichun;Wang, Xiling;Tang, Xing;Yu, Maode;Li, Zhengang;Huang, Ping

作者机构：

关键词： Mulberry;Flavonoids;Chalcone synthase;Stilbene synthase;Co-expression;Transgenic

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：4.27； 五年影响因子：4.816 ）

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年卷期：

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收录情况： SCI

摘要： Chalcone synthase (CHS) is the pivotal enzyme that catalyzes the first committed step of the phenyl-propanoid pathway leading to flavonoids. Here, five CHS genes were determined in mulberry (Morus atropurpurea Roxb.). Interestingly, phylogenetic analysis tended to group three MaCHSs in the stilbene synthase (STS) family and initially annotated these as MaSTSs. A co-expression system that harbored a 4-coumarate:CoA ligase gene and one of the candidate genes was established to determine the functions of this novel gene family. The fermentation result demonstrated that MaSTS in fact encoded a CHS enzyme, and was consequently retermed MaCHS. Tissue-specific expression analysis indicated that MaCHS1/MaCHS2 was highly abundant in fruit, and MaCHS4 had significant expression in root bark, stem bark and old leaves, while MaCHS3 and MaCHS5 were more expressed in old leaves. Subcellular localization experiments showed that MaCHS was localized to the cytoplasm. Transcription levels suggested MaCHS genes were involved in a series of defense responses. Over-expression of MaCHS in transgenic tobacco modified the metabolite profile, and resulted in elevated tolerance to a series of environmental stresses. This study comprehensively evaluated the function of MaCHS genes and laid the foundation for future research on MaCHS in mulberry. (C) 2017 Elsevier Masson SAS. All rights reserved.

分类号： Q945`Q946

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