Genomic and phenotypic analyses of Pseudomonas psychrotolerans PRS08-11306 reveal a turnerbactin biosynthesis gene cluster that contributes to nitrogen fixation

文献类型: 外文期刊

第一作者: Liu, Ruifang

作者: Liu, Ruifang;Zhang, Yu;Chen, Ping;Lin, Haiyan;Ye, Guoyou;Wang, Zhongwei;Ge, Changwei;Ren, Deyong;Liu, Ruifang;Lin, Haiyan;Zhu, Bo;Zhu, Bo

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关键词: Pseudomonas psychrotolerans;Turnerbactin;PacBio;Siderophore;Nitrogen fixation

期刊名称:JOURNAL OF BIOTECHNOLOGY ( 影响因子:3.307; 五年影响因子:3.778 )

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收录情况: SCI

摘要: Plant-microbe interactions can provide agronomic benefits, such as enhancing nutrient uptake and providing fixed nitrogen. The Pseudomonas psychrotolerans strain PRS08-11306 was isolated from rice seeds and can enhance plant growth. Here, we analyzed the P. psychrotolerans genome, which is similar to 5 Mb, with 4389 coding sequences, 77 tRNAs, and 7 rRNAs. Genome analysis identified a cluster of turnerbactin biosynthetic genes, which are responsible for the production of a catecholate siderophore and contribute to nitrogen fixation for the host. Analysis of the transcription factor mutant Delta rpoS, which does not express this gene cluster, confirmed the relationship between the gene cluster and siderophore production. The nitrogen fixation characteristics of the cluster were confirmed in a plant growth-promoting experiment. The annotated full genome sequence of this strain sheds light on the role of P. psychrotolerans PRS08-11306 as a plant beneficial bacterium.

分类号: Q81

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