Extraction optimization, preliminary characterization and antioxidant activities of polysaccharides from Glycine soja

文献类型: 外文期刊

第一作者: Jing, Changliang

作者: Jing, Changliang;Yuan, Yuan;Tang, Qi;Zou, Ping;Li, Yiqiang;Zhang, Chengsheng

作者机构:

关键词: Glycine soja;Polysaccharide fractions;Preliminary structural characterization;Antioxidant activity

期刊名称:INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES ( 影响因子:6.953; 五年影响因子:6.737 )

ISSN:

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收录情况: SCI

摘要: Single-factor experiment and Central Composite Design (CCD) was applied to optimize the ultrasound assisted extraction (UAE) conditions of polysaccharides from Glycine soja (CGPS), and a preliminary characterization of three polysaccharide fractions (CGPS, GPS-1, and GPS-2) and their antioxidant activities were investigated. Under the optimal conditions: ratio of liquid to solid 42.7 mL/g, extraction power 293.7 W, extraction temperature 68.9 degrees C, and extraction time 34.7 min, the experimental CGPS yield was 6.04 mg/g. CGPS was further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions (GPS-1 and GPS-2), and their monosaccharides compositions were characterized by HPLC. Fourier-transform infrared spectra (FT-IR) indicated the chemical structures of them. Moreover, they exhibited high antioxidant activities in a concentration-dependent manner in vitro. In summary, the present study suggested that UAE was a very effective method to extract polysaccharides from Glycine soja and the polysaccharides could be explored as potential antioxidant agents for medicine and function food. (C) 2017 Elsevier B.V. All rights reserved.

分类号: Q71

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