An ultrasensitive gray-imaging-based quantitative immunochromatographic detection method for fumonisin B-1 in agricultural products

文献类型： 外文期刊

第一作者： Tang, Xiaoqian

作者： Tang, Xiaoqian;Li, Peiwu;Zhang, Zhaowei;Zhang, Qi;Guo, Jingnan;Zhang, Wen;Tang, Xiaoqian;Li, Peiwu;Zhang, Zhaowei;Zhang, Qi;Zhang, Wen;Tang, Xiaoqian;Li, Peiwu;Zhang, Zhaowei;Zhang, Qi;Zhang, Wen;Tang, Xiaoqian;Li, Peiwu;Zhang, Qi;Zhang, Wen;Tang, Xiaoqian;Li, Peiwu;Zhang, Qi;Zhang, Wen

作者机构：

关键词： Fumonisin B-1;Monoclonal antibody;Gray imaging;Immunoassay;Agricultural product

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Fumonisins (FBs) are widely found in rice, maize, peanut, wheat, and other agricultural products. These have been detected using a chromatography technique, whereas the rapid assay by a highly sensitive monoclonal antibody is minimally reported. Herein, a highly sensitive monoclonal antibody (7A11) was successfully developed by hybridoma technique. The 50% inhibition concentration (IC50) of 7A11 monoclonal antibody was 0.32 ng/mL in an optimized buffer. The cross-reactivity between fumonisin B-1 and fumonisin B-2, B-3 was 4.3% and 12.8%, respectively. Based on the newly developed 7A11 antibody, a high sensitivity indirect competitive enzyme-linked immunosorbent assay (icELISA) and gold nanoparticles-based gray imaging quantification immunoassay (GNPs-GI) were established. The analytical range of icELISA was 0.08-1.38 ng/mL and that for GNPs-GI was 0.24-15 ng/ml. Both the methods showed adequate recoveries (80.0-105.8% for icELISA and 78.5-115.2% for GNPs-GI) in spiked samples. Compared to high-performance liquid chromatography (HPLC), icELISA and GNPs-GI indicated reliability that could be used for further detection of fumonisin B-1 in agricultural products. (C) 2017 Elsevier Ltd. All rights reserved.

分类号： S7

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