PA-X protein decreases replication and pathogenicity of swine influenza virus in cultured cells and mouse models

文献类型: 外文期刊

第一作者: Gong, Xiao-Qian

作者: Gong, Xiao-Qian;Ruan, Bao-Yang;Liu, Xiao-Min;Wang, Qi;Yang, Hai-Ming;Wang, Shuai-Yong;Zhang, Peng;Wang, Xiu-Hui;Shan, Tong-Ling;Tong, Wu;Zhou, Yan-Jun;Li, Guo-Xin;Zheng, Hao;Tong, Guang-Zhi;Yu, Hai;Sun, Ying-Feng;Shan, Tong-Ling;Tong, Wu;Zhou, Yan-Jun;Li, Guo-Xin;Zheng, Hao;Tong, Guang-Zhi;Yu, Hai

作者机构:

关键词: Swine influenza virus;Pa-x;Pathogenicity;Virus replication;Polymerase activity

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Swine influenza viruses have been circulating in pigs throughout world and might be potential threats to human health. PA-X protein is a newly discovered protein produced from the PA gene by ribosomal frameshifting and the effects of PA-X on the 1918 H1N1, the pandemic 2009 H1N1, the highly pathogenic avian H5N1 and the avian H9N2 influenza viruses have been reported. However, the role of PA-X in the pathogenesis of swine influenza virus is still unknown. In this study, we rescued the H1N1 wild-type (WT) classical swine influenza virus (A/Swine/Guangdong/1/2011 (H1N1)) and H1N1 PA-X deficient virus containing mutations at the frameshift motif, and compared their replication properties and pathogenicity of swine influenza virus in vitro and in vivo. Our results show that the expression of PA-X inhibits virus replication and polymerase activity in cultured cells and decreases virulence in mouse models. Therefore, our study demonstrates that PA-X protein acts as a negative virulence regulator for classical H1N1 swine influenza virus and decreases virulence by inhibiting viral replication and polymerase activity, deepening our understanding of the pathogenesis of swine influenza virus.

分类号: S9

  • 相关文献

[1]Voltage-Dependent Anion Channel 1 Interacts with Ribonucleoprotein Complexes To Enhance Infectious Bursal Disease Virus Polymerase Activity. Han, Chunyan,Yao, Shuai,Gao, Li,Zhang, Lizhou,Qi, Xiaole,Duan, Yulu,Yang, Bo,Gao, Yulong,Liu, Changjun,Zhang, Yanping,Wang, Yongqiang,Wang, Xiaomei,Han, Chunyan,Zeng, Xiangwei,Yao, Shuai,Yang, Bo.

[2]Impacts of different expressions of PA-X protein on 2009 pandemic H1N1 virus replication, pathogenicity and host immune responses. Leea, Jinhwa,Yua, Hai,Li, Yonghai,Ma, Jingjiao,Lang, Yuekun,Duff, Michael,Henningson, Jamie,Liu, Qinfang,Li, Yuhao,Nagy, Abdou,Bawa, Bhupinder,Richt, Juergen A.,Ma, Wenjun,Yua, Hai,Liu, Qinfang,Li, Zejun,Tong, Guangzhi,Nagy, Abdou.

[3]Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus. Liu, Huili,Tao, Jie,Zhang, Pengchao,Yin, Xiuchen,Ha, Zhuo,Zhang, Chunling,Liu, Huili,Tao, Jie,Yin, Xiuchen.

[4]Interactomic landscape of PA-X-chicken protein complexes of H5N1 influenza A virus. Li, Qinghe,Yuan, Xiaoya,Wang, Qiao,Wang, Fei,Liu, Ranran,Zheng, Maiqing,Wen, Jie,Zhao, Guiping,Yuan, Xiaoya,Chang, Guobin,Chen, Guohong.

[5]TMPRSS2 and MSPL Facilitate Trypsin-Independent Porcine Epidemic Diarrhea Virus Replication in Vero Cells. Shi, Wen,Fan, Wenlu,Bai, Jing,Wang, Li,Jiang, Yanping,Tang, Lijie,Cui, Wen,Xu, Yigang,Li, Yijing,Tang, Yandong,Liu, Min. 2017

[6]The roles of ascorbic acid and glutathione in symptom alleviation to SA-deficient plants infected with RNA viruses. Xu, Fei,Shang, Jing,Xu, Mo-Yun,Wang, Jian-Hui,Xi, De-Hui,Zhu, Feng,Yuan, Shu,Yang, Hui,Jia, Shu-Dan,Zhang, Zhong-Wei,Lin, Hong-Hui,Wang, Jian-Hui.

[7]Autophagy in Negative-Strand RNA Virus Infection. Wang, Yupeng,Jiang, Ke,Meng, Songshu,Zhang, Quan,Ding, Chan. 2018

[8]Host range expansion of honey bee Black Queen Cell Virus in the bumble bee, Bombus huntii. Boncristiani, Humberto,Hamilton, Michele,Chen, Yanping,Peng, Wenjun,Li, Jilian,Strange, James P..

[9]Overexpression of microRNA gga-miR-21 in chicken fibroblasts suppresses replication of infectious bursal disease virus through inhibiting VP1 translation. Ouyang, Wei,Pan, Qun-xing,Wang, Xiao-li,Xia, Xing-xia,Bi, Zhen-wei,Wang, Yong-qiang,Wang, Xiao-mei.

[10]Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons. Li, Liwei,Zhou, Yanjun,Gao, Fei,Jiang, Yifeng,Yu, Lingxue,Zheng, Hao,Tong, Wu,Yang, Shen,Zheng, Haihong,Shan, Tongling,Liu, Fei,Xia, Tianqi,Tong, Guangzhi,Wei, Zuzhang,Zhou, Yanjun,Gao, Fei,Tong, Guangzhi.

[11]T135I substitution in the nonstructural protein 2C enhances foot-and-mouth disease virus replication. Yuan, Tiangang,Wang, Haiwei,Li, Chen,Yang, Decheng,Zhou, Guohui,Yu, Li.

[12]Pseudorabies viral replication is inhibited by a novel target of miR-21. Huang, Jing,Fu, Liangliang,Jia, Hao,Zhu, Mengjin,Li, Xinyun,Zhao, Shuhong,Huang, Jing,Ma, Guojian.

[13]Effective inhibition of infectious bursal disease virus replication in vitro by DNA vector-based RNA interference. Gao, Yulong,Liu, Wei,Gao, Honglei,Qi, Xiaole,Lin, Huan,Wang, Xiaomei,Shen, Rongxian,Gao, Yulong,Wang, Xiaomei. 2008

[14]Cis-acting structural element in 5 ' UTR is essential for infectivity of porcine reproductive and respiratory syndrome virus. Gao, Fei,Yao, Huochun,Yang, Qian,Gao, Fei,Lu, Jiaqi,Wei, Zuzhang,Yuan, Shishan. 2012

[15]Genomic Analysis of One Chinese H1N1 Swine Influenza Virus Strain from Healthy Pig Remaining Different Virulence Determinants. Xu Cong,Xue Chun-Yi,Lv Li-Shan,Li Xiao-Ming,Cao Yong-Chang,Shu Ding-Ming,Lin Zhi-Xiong. 2012

[16]The effects of H3N2 swine influenza virus infection on TLRs and RLRs signaling pathways in porcine alveolar macrophages. Zhang, Jinqiu,Hou, Jibo,Zhang, Jinqiu,Miao, Jinfeng,Lu, Chengping. 2015

[17]A recombinant pseudorabies virus encoding the HA gene from H3N2 subtype swine influenza virus protects mice from virulent challenge. Tian, ZJ,Zhou, GH,Zheng, BL,Qiu, HJ,Ni, JQ,Yang, HL,Yin, XN,Hu, SP,Tong, GZ. 2006

[18]Immune efficacy of an adenoviral vector-based swine influenza vaccine against antigenically distinct H1N1 strains in mice. Wu, Yunpu,Wei, Ping,Wu, Yunpu,Yang, Dawei,Xu, Bangfeng,Liang, Wenhua,Sui, Jinyu,Chen, Yan,Yang, Huanliang,Chen, Hualan,Qiao, Chuanling. 2017

[19]Novel triple reassortant H1N2 influenza viruses bearing six internal genes of the pandemic 2009/H1N1 influenza virus were detected in pigs in China. Qiao, Chuanling,Liu, Liping,Yang, Huanliang,Chen, Yan,Xu, Huiyang,Chen, Hualan.

[20]Protective efficacy of a high-growth reassortant swine H3N2 inactivated vaccine constructed by reverse genetic manipulation. Wen, Feng,Ma, Ji-Hong,Yu, Hai,Yang, Fu-Ru,Huang, Meng,Zhou, Yan-Jun,Li, Ze-Jun,Tong, Guang-Zhi.

作者其他论文 更多>>