M protein is sufficient for assembly and release of Peste des petits ruminants virus-like particles

文献类型： 外文期刊

第一作者： Wang, Qiuxia

作者： Wang, Qiuxia;Ou, Changbo;Liu, Xingyou;Yu, Yan;Jiang, Jinqing;Ma, Jinyou;Hu, Jianhe;Wang, Qiuxia;Dou, Yongxi;Chen, Lei;Meng, Xuelian;Zhang, Zhidong;Cai, Xuepeng;Cai, Xuepeng

作者机构：

关键词： Peste des petits ruminants virus;Virus-like particles;M protein;Assembly;Release

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Peste des petits ruminants virus (PPRV), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (M), nucleocapsid proteins (N), fusion protein (F) and hemagglutinin protein (H)) and could cause high morbidity and mortality in sheep and goats. Although a vaccine strain of PPRV has been rescued and co-expression of M and N could yield PPRV-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. In this study, plasmids carrying PPRV cDNA sequences encoding the N, M, H, and F proteins were expressed in Vero cells. The co-expression of all four proteins resulted in the release of virus-like particles (VLPs) with similar release efficiency to that of authentic virions. Moreover, the co-expression of M together with F also resulted in efficient VLPs release. In the absence of M protein, the expression of no combination of the other proteins resulted in particle release. In summary, a VLPs production system for PPRV has been established and M protein is necessary for promoting the assembly and release of VLPs, of which the predominant protein is M protein. Further study will be focused on the immunogenicity of the VLPs. (C) 2017 Published by Elsevier Ltd.

分类号： S85

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