Isolation, pathogenicity and characterization of a novel bacterial pathogen Streptococcus uberis from diseased mandarin fish Siniperca chuatsi

文献类型： 外文期刊

第一作者： Luo, Xia

作者： Luo, Xia;Fu, Xiaozhe;Liao, Guoli;Chang, Ouqin;Huang, Zhibin;Li, Ningqiu;Fu, Xiaozhe;Li, Ningqiu

作者机构：

关键词： Mandarin fish;Streptococcus uberis;Pathogenicity;16S rRNA;GapC;Histopathology

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In recent years, mandarin fish had a high mortality rate associated with abnormal swimming, exophthalmia, corneal opacity and eye hemorrhage on a fish farm located at Foshan city, Guangdong province, China. Three isolates of Gram-positive, chain-forming cocci were recovered from moribund fish, and designated as SS131025-1, SS131025-2, and SS131025-3. These isolates were identified as Streptococcus uberis according to their morphologic and physio-biochemical characteristics as well as phylogenetic analysis based on their 16S rRNA and GapC gene sequences. The pathogenicity of S. uberis to mandarin fish was determined by challenge experiments. Results of artificial challenge showed S. uberis infected healthy mandarin fish and lead to death by eyeball injection or immersion route, and the LD50 of SS131025-1 with eyeball injection was 2.0 x 10(6.42) CFU per fish. Moreover extracellular product (ECP) of the isolated S.uberis induced CPB cell apoptosis and cause death of mandarin fish. In addition, these S. uberis strains could also infect tilapia, but not grass carp and crucian carp, and grew in brain-heart infusion broth with an optimal temperature of 37 degrees C, pH of 7.0, and salinity of 0%. Antibiotic sensitivity testing indicated that these isolates were susceptible to rifampicin and furazolidone but resistant to 20 kinds of antibiotics. Histopathologically, infection with S. uberis could cause serious pathological changes in brain tissues such as vacuoles in matrix, swollen mitochondria with lysis of cristae and disintegration, and lots of coccus was observed both under electron and light microscope. These results shed some light on the pathogenicity of the isolates and how to prevent and control S. uberis infection in mandarin fish. (C) 2017 Elsevier Ltd. All rights reserved.

分类号： S85

  • 相关文献

[1]Nocardia seriolae infection in cultured jade perch, Scortum barcoo. Wang, Fang,Liu, Chun,Chang, Ouqin,Feng, Yongyong,Jiang, Lang,Li, Kaibin,Wang, Xuguang. 2017

[2]The effect of taurine on the toll-like receptors/nuclear factor kappa B (TLRs/NF-kappa B) signaling pathway in Streptococcus uberis-induced mastitis in rats. Miao, Jinfeng,Zheng, Liuhai,Ma, Zili,Zhu, Wei,Zou, Sixiang,Zhang, Jinqiu. 2011

[3]The role of NADPH oxidase in taurine attenuation of Streptococcus uberis-induced mastitis in rats. Miao, Jinfeng,Ma, Zili,Zheng, Liuhai,Zhang, Jinqiu. 2013

[4]The role of Ca2+ mediated signaling pathways on the effect of taurine against Streptococcus uberis infection. Dai, Bin,Liu, Ming,Zhang, Yuanshu,Xu, Yuanyuan,Miao, Jinfeng,Zhang, Jinqiu,Lu, Jinye,Yin, Yulong.

[5]Taurine attenuates Streptococcus uberis-induced mastitis in rats by increasing T regulatory cells. Miao, Jinfeng,Zheng, Liuhai,Yu, Xiaoming,Zhu, Wei,Zou, Sixiang,Zhang, Jinqiu.

[6]Susceptibility of Chinese Perch Brain (CPB) Cell and Mandarin Fish to Red-Spotted Grouper Nervous Necrosis Virus (RGNNV) Infection. Tu, Jiagang,Chen, Wenjie,Zhao, Lijuan,Lan, Jiangfeng,Lin, Li,Fu, Xiaozhe,Lin, Qiang,Chang, Ouqin,Li, Ningqiu. 2016

[7]Protective immunity against iridovirus disease in mandarin fish, induced by recombinant major capsid protein of infectious spleen and kidney necrosis virus. Fu, Xiaozhe,Li, Ningqiu,Lai, Yingtiao,Liu, Lihui,Lin, Qiang,Shi, Cunbin,Huang, Zhibin,Wu, Shuqin. 2012

[8]Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV). Fu, Xiaozhe,Li, Ningqiu,Liu, Lihui,Lin, Qiang,Wang, Fang,Lai, Yingtiao,Jiang, Haiming,Pan, Houjun,Shi, Cunbin,Wu, Shuqin. 2011

[9]Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation. Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,Xu, Z.,Yao, W. J.,Nie, P.,Tian, J. Y.,Xie, H. X.,Zhang, Y. A.,Xu, Z.,Yao, W. J.,Nie, P.,Tian, J. Y..

[10]Investigating the potential immune role of fish NCAMs: Molecular cloning and expression analysis in mandarin fish. Wu, Nan,Zhang, Xiang-Yang,Zhang, Nu,Zhang, Xu-Jie,Guo, Xia,Chen, Xiao-Ling,Zhang, Yu,Wu, Han,Li, Shun,Li, Ai-Hua,Zhang, Yong-An,Zhang, Xiang-Yang,Zhang, Nu,Huang, Bei,Zhang, Xu-Jie,Guo, Xia.

[11]Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations. Hou, Liping,Yang, Yang,Shu, Hu,Cheng, Xuemei,Xu, Hongyan,Ying, Guangguo,Zhao, Jianliang,Ying, Guangguo,Zhao, Jianliang. 2017

[12]Edwardsiellosis in farmed turbot, Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical, aetiological and histopathological study. Qin, L.,Xu, J.,Wang, Y. G.. 2014

[13]Histopathological Liver and Testis Alterations in Male Half Smooth Tongue Sole (Cynoglossus semilaevis) Exposed to Endocrine Disruptors. Li, Fengling,Yao, Lin,Sun, Weihong,Jiang, Yanhua,Li, Zhaoxin,Zhai, Yuxiu. 2017

[14]Recovery and pathogenicity analysis of Aerococcus viridans isolated from tilapia (Orecohromis niloticus) cultured in southwest of China. Ke, Xiaoli,Lu, Maixin,Ye, Xing,Gao, Fengying,Zhu, Huaping,Huang, Zhanghan. 2012

[15]Action of nitromezuril against Eimeria tenella with clinically anticoccidial indices and histopathology. She, Rufeng,Fei, Chenzhong,Chen, Huiya,Wang, Xiaoyang,Wang, Mi,Zhang, Keyu,Zhang, Lifang,Wang, Chunmei,Liu, Yingchun,Zheng, Wenli,Xue, Feiqun.

[16]Experimental inoculation of oriental river prawn Macrobrachium nipponense with white spot syndrome virus (WSSV). Zhao, Caiyuan,Fu, Hongtuo,Fu, Hongtuo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Gong, Yongsheng.

[17]Thelohanellus wangi n. sp (Myxozoa, Myxosporea), a new gill parasite of allogynogenetic gibel carp (Carassius auratus gibelio Bloch) in China, causing severe gill myxosporidiosis. Yuan, S.,Wang, J. G.,Zhang, J. Y.,Yuan, S.,Xi, B. W.,Xie, J..

[18]Toxicity of Dietary Melamine to Laying Ducks: Biochemical and Histopathological Changes and Residue in Eggs. Gao, Chun-Qi,Liu, Fu-Zhu,Gao, Chun-Qi,Wu, Shu-Geng,Yue, Hong-Yuan,Ji, Feng,Zhang, Hai-Jun,Liu, Qing-Sheng,Fan, Zhi-Ying,Qi, Guang-Hai.

[19]Immunotoxicity assessment of cadinene sesquiterpenes from Eupatorium adenophorum in mice. Liu Xiao-man,Yan Dong-dong,Li Yuan,Wang Qiu-xia,Cao Ao-cheng,Guo Mei-xia. 2016

[20]Increased survival and reduced renal injury in MRL/lpr mice treated with a human Fc? receptor II (CD32) peptide. Xi, Jun,Zhang, Gai P.,Qiao, Song L.,Guo, Jun Q.,Yang, Yan Y.,Zhang, Li N.,Miao, Xian W.,Zhao, Dong,Zhi, Yu B.,Cai, Shu J.,Luo, Jun,Deng, Rui G.,Xi, Jun,Wang, Xuan N.. 2012

作者其他论文 更多>>

微信小程序