Production and Identification of a Tetraploid Germplasm of Edible Lilium davidii var. unicolor Salisb via Chromosome Doubling

文献类型： 外文期刊

第一作者： Feng, Yayan

作者： Feng, Yayan;Xu, Leifeng;Yang, Panpan;Xu, Hua;Cao, Yuwei;Tang, Yuchao;Yuan, Suxia;Ming, Jun;Yang, Panpan

作者机构：

关键词： edible lily;chromosome doubling;colchicine;oryzalin;tetraploid

期刊名称：HORTSCIENCE （ 影响因子：1.455； 五年影响因子：1.617 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Lilium davidii var. unicolor Salisb is a cultivar of Lilium (Liliaceae) with important edible and ornamental characteristic. The application and production of Lilium davidii var. unicolor Salisb were still facing large problems because of its several disadvantages such as narrow range of adaptability, small annual growth increment, and low fertility. To achieve broader environmental adaptability and obtain a more nutritious germplasm, we used colchicine and oryzalin to induce chromosome doubling via the soaking method. Tissue culture bulbs were treated with colchicine at 0.03%, 0.05%, or 0.08% for 32, 40, or 48 hours or with oryzalin at 0.002%, 0.005%, 0.008%, or 0.01% for 3, 6, 9, 12, or 24 hours before being transferred to a differentiating medium. The results showed that colchicine treatment resulted in the highest induction rate when applied at 0.05% for 48 hours, whereas oryzalin treatment produced fewer tetraploid plants. The chromosome number of induced plants with small stoma density and longer guard cells is twice than that of the diploid. The plants were identified as tetraploid. In this study, a new germplasm of Lilium davidii var. unicolor Salisb was innovative and showed novel genetic characteristic.

分类号： S6

  • 相关文献

[1]Enhancement of artemisinin content in tetraploid Artemisia annua plants by modulating the expression of genes in artemisinin biosynthetic pathway. Lin, Xiuyan,Lu, Xu,Zhang, Fangyuan,Shen, Qian,Wu, Shaoyan,Chen, Yunfei,Wang, Tao,Tang, Kexuan,Zhou, Yin,Zhang, Jianjun.

[2]Generation of octoploid switchgrass in three cultivars by colchicine treatment. Yue, Yuesen,Zhu, Yi,Fan, Xifeng,Hou, Xincun,Zhao, Chunqiao,Zhang, Shuang,Wu, Juying.

[3]A Series of Polyploid Grape Cultivars and Their Structural Identification of Ploidy Character. Tang, X. P.,Chen, J.,Ma, X. H.,Dong, Z. G.,Zhao, Q. F.,Li, X. M.,Tan, W..

[4]In rice, Oryzalin and abscisic acid differentially affect tubulin mRNA and protein levels. Giani, S,Qin, XQ,Faoro, F,Breviario, D. 1998

[5]Overcoming obstacles to interspecific hybridization between Gossypium hirsutum and G. turneri. Chen, Yu,Chen, Yu,Feng, Shouli,Zhao, Ting,Zhou, Baoliang. 2018

[6]Chromosome Doubling of Microspore-Derived Plants from Cabbage (Brassica oleracea var. capitata L.) and Broccoli (Brassica oleracea var. Italica L.). Yuan, Suxia,Su, Yanbin,Liu, Yumei,Li, Zhansheng,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao,Sun, Peitian. 2015

[7]Production and characterization of an amphidiploid derived from interspecific hybridization between Solanum melongena L. and Solanum aculeatissimum Jacq.. Zhou, Xiaohui,Bao, Shengyou,Liu, Jun,Yang, Yan,Zhuang, Yong. 2018

[8]Breeding of newly licensed wheat variety Huapei 8 and improved breeding strategy by anther culture. Kang Ming-hui,Hai Yan,Huang Bing-yan,Zhao Yong-ying,Wang Shi-jie,Miao Li-juan,Zhang Xin-you. 2011

[9]Induction and identification of Stylosanthes guianensis tetraploids. Wu, F. H.,Yu, X. D.,Zhuang, N. S.,Liu, J. P.,Liu, G. D.. 2015

[10]Effects of different states of sheep fetal fibroblasts as donor cells on the early development in vitro of reconstructed sheep embryos. Wang Hai,Ao Hong,Pan QiuZhen,Li RongQi,Zhao MengBin,Lian ZhengXing,Li Ning,Wu ChangXin.

[11]Anther culture in connection with induced mutations for rice improvement. Chen, QF,Wang, CL,Lu, YM,Shen, M,Afza, R,Duren, MV,Brunner, H. 2001

[12]In vitro induction of tetraploids in cassava variety 'Xinxuan 048' using colchicine. Zeng, Wen-dan,Yan, Hua-bing.

[13]Identification and Evaluation of Single-Nucleotide Polymorphisms in Allotetraploid Peanut (Arachis hypogaea L.) Based on Amplicon Sequencing Combined with High Resolution Melting (HRM) Analysis. Hong, Yanbin,Liu, Ying,Chen, Xiaoping,Liang, Xuanqiang,Hong, Yanbin,Huang, Shangzhi,Pandey, Manish K.,Varshney, Rajeev K.,Liu, Hong,Varshney, Rajeev K.,Varshney, Rajeev K.. 2015

[14]Complete mitochondrial DNA genome of tetraploid Carassius auratus gibelio. Li, Zhong,Liang, Hong-Wei,Zou, Gui-Wei. 2016

[15]Ploidy Variation in Hemerocallis spp. and the Implications on Daylily Breeding. Zhang, C.,Cao, D. M.,Kang, L. F.,Duan, J. J.,Ma, X. L.,Yan, G. J.,Wang, Y. S.,Zhang, X. C.. 2013

[16]Morphological, cytological and fertility consequences of a spontaneous tetraploid of the diploid pear (Pyrus pyrifolia Nakai) cultivar 'Cuiguan'. Wang, Xiaoqing,Shi, Chunhui,Zhang, Xueying,Duan, Ke,Luo, Jun,Wang, Xiaoqing,Shi, Chunhui,Zhang, Xueying,Duan, Ke,Luo, Jun,Wang, Haihai.

[17]Isolation, molecular characterization, and mapping of four rose MLO orthologs. Kaufmann, Helgard,Wehmeyer, Juliane,Debener, Thomas,Qiu, Xianqin. 2012

[18]Heteroallelic diploid pollen led to self-compatibility in tetraploid cultivar 'Sha 01' (Pyrus sinkiangensis Yu). Qi, Yong-Jie,Wu, Hua-Qing,Wu, Jun,Tao, Shu-Tian,Zhang, Shao-Ling,Cao, Yu-Fen. 2011

[19]Generation of a New Polyploid Grape Cultivar by Using Hybrid Seeds Induced with Colchicine. Chen, Jun,Tang, Xiaoping,Ma, Xiaohe,Zhao, Qifeng,Dong, Zhigang.

[20]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

作者其他论文 更多>>

微信小程序