Synbiotic dietary supplement affects growth, immune responses and intestinal microbiota of Apostichopus japonicus

文献类型： 外文期刊

第一作者： Wang, Xitao

作者： Wang, Xitao;Wang, Lili;Li, Xiaoyu;Qu, Kunli;Xu, Yongping;Sun, Yongxin;Li, Xiaoyu;Xu, Yongping

作者机构：

关键词： Synbiotic;Probiotic;Prebiotic;Apostichopus japonicus;Intestinal microbiome

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A feeding experiment was conducted to investigate the effects of dietary administration of synbiotic with Bacillus lincheniformis WS-2 (CGMCC No. 12813) and alginate oligosaccharides (AOS) on the growth, innate immune response, and intestinal microbiota of the sea cucumber Apostichopus japonicus and its resistance to Vibrio infection. Sea cucumbers were given a control diet (non-supplemented), pro diet (basal diet plus 1 x 10(9) cfu (g diet)(-1) B. lincheniformis WS-2), syn diet (basal diet plus 1 x 10(9) cfu (g diet)(-1) B. lincheniformis WS-2 and 10 g (kg diet)(-1) AOS) or pre diet (basal diet plus 10 g (kg diet)(-1) AOS) over a period of 60 days, and the growth performance and various innate immune parameters of the animals were evaluated after 30 and 60 days of feeding. No significant difference in growth performance was observed between the group fed with the syn and the group fed with the pro diet, but both these groups exhibited significant (P < 0.05) enhancement in growth performance compared to the control group. At the same time, both syn and pro diets also resulted in the animals having significantly higher levels of amylase, protease and alginate lyase activities compared to the con diet. Individuals fed with the syn or pro diet showed enhanced levels of various immune enzyme activities, compared to those fed with the con diet. At the end of the growth period, the sea cucumbers were challenged with Vibrio splendidus via intraperitoneal injection. The survival rates of sea cucumbers fed with the syn, pro or pre diet were significantly improved compared to that of sea cucumbers fed with the con diet, with sea cucumbers fed with synbiotic having the highest survival. In addition, increased proportions of Bacillus and Lactococcus were found in the intestinal tract of sea cucumbers fed with the syn diet (9.5% and 7.3%) compared to those of sea cucumbers fed with the pro diet (6.1% and 4.6%), con diet (4.0% and 3.4%), or pre diet (5.2% and 6.8%) after 60 days of feeding. Furthermore, the proportion of Vibrio in the intestinal tracts of sea cucumbers fed with the pro diet (2%) or syn diet (3.1%) was lower than that of sea cucumbers fed with the con diet (5.5%) or pre diet (3.8%), although no significant difference was detected between the pro diet and syn diet groups (P > 0.05). Overall, the results suggested that dietary synbiotic consisting of Bacillus lincheniformis and alginate oligosaccharides (AOS) could have positive benefit for sea cucumber aquaculture. (C) 2017 Published by Elsevier Ltd.

分类号： S9

  • 相关文献

[1]Effects of dietary chitosan oligosaccharides and Bacillus coagulans on the growth, innate immunity and resistance of koi (Cyprinus carpio koi). Lin, Shimei,Mao, Shuhong,Guan, Yong,Luo, Li,Pan, Yu,Lin, Shimei,Luo, Li,Luo, Lin. 2012

[2]Prebiotic Potential of Xylooligosaccharides Derived from Corn Cobs and Their In Vitro Antioxidant Activity When Combined with Lactobacillus. Yu, Xiuhua,Yin, Jianyuan,Zhao, Chunfang,Yu, Xiuhua,Li, Lin,Luan, Chang,Zhang, Jian,Li, Shengyu.

[3]Effects of dietary supplementation of synbiotics on growth performance, intestinal morphology, sIgA content and antioxidant capacities of broilers. Min, Y. N.,Yang, H. L.,Gao, Y. P.,Xu, Y. X..

[4]Dietary High Zinc Oxide Modulates the Microbiome of Ileum and Colon in Weaned Piglets. Yu, Ting,Zhu, Cui,Feng, Ruowei,Zhu, Qingfeng,Xu, Jinsong,Chen, Zhuang,Jiang, Zongyong,Chen, Shicheng,Gao, Lei,Lv, Hang,Jiang, Zongyong. 2017

[5]Utility of Thermostable Xylanases of Mycothermus thermophilus in Generating Prebiotic Xylooligosaccharides. Ma, Rui,Bai, Yingguo,Huang, Huoqing,Luo, Huiying,Yao, Bin,Ma, Rui,Fan, Yunliu,Ma, Rui,Chen, Sanfeng,Cai, Lei.

[6]Effects of soybean oligosaccharides on intestinal microbial communities and immune modulation in mice. Ma, Yan,Meng, Xianjun,Wu, Xingzhuang,Giovanni, Vigna. 2017

[7]Antioxidant activity of prebiotic ginseng polysaccharides combined with potential probiotic Lactobacillus plantarum C88. He, Zhongmei,Wang, Xiaohui,Wang, Xiaohui,Zhao, Yujuan,Zhang, Jian,Niu, Chunhua,Li, Shengyu,Li, Guofeng,Ying, Dashi,Zhang, Li,Zhang, Xue.

[8]Effects of dietary poly-beta-hydroxybutyrate (PHB) on microbiota composition and the mTOR signaling pathway in the intestines of litopenaeus vannamei. Duan, Yafei,Zhang, Yue,Dong, Hongbiao,Wang, Yun,Zhang, Jiasong. 2017

[9]1-Deoxynojirimycin from Bacillus subtilis improves antioxidant and antibacterial activities of juvenile Yoshitomi tilapia. Tang, Lining,Huang, Kai,Sun, Lei,Huang, Qing,Bi, Yanjun,Xie, Jun,Yu, Dan. 2017

[10]The ability of marine Bacillus spp. isolated from fish gastrointestinal tract and culture pond sediment to inhibit growth of aquatic pathogenic bacteria. Chen, Y.,Zhu, W.,Li, J.,Mo, Z.,Xiao, P.. 2016

[11]The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Deng, W.,Dong, X. F.,Tong, J. M.,Zhang, Q.. 2012

[12]Identification and characterization of Bacillus subtilis from grass carp (Ctenopharynodon idellus) for use as probiotic additives in aquatic feed. Guo, Xia,Chen, Dan-Dan,Peng, Kai-Song,Cui, Zheng-Wei,Zhang, Xu-Jie,Li, Shun,Zhang, Yong-An,Guo, Xia,Peng, Kai-Song,Cui, Zheng-Wei,Zhang, Xu-Jie.

[13]Effect of Bacillus cereus as a water or feed additive on the gut microbiota and immunological parameters of Nile tilapia. Wang, Miao,Lu, Maixin,Ke, Xiaoli,Liu, Zhigang,Gao, Fengying,Cao, Jianmeng,Zhu, Huaping,Yi, Mengmeng,Yu, Deguang,Liu, Guanbin.

[14]Isolation and characterization of Bacillus spp. M001 for potential application in turbot (Scophthalmus maximus L.) against Vibrio anguillarum. Chen, Y.,Zhu, W. Y.,Li, J.,Li, G. Y.,Huang, J.,Mo, Z. L.,Xiao, P.,Yue, S..

[15]EPSP of L. casei BL23 Protected against the Infection Caused by Aeromonas veronii via Enhancement of Immune Response in Zebrafish. Qin, Chubin,Wang, Yibing,Li, Weifen,Qin, Chubin,Zhang, Zhen,Wang, Yibing,Li, Shuning,Ran, Chao,Hu, Jun,Xie, Yadong,Zhou, Zhigang. 2017

[16]Cellulose-Based Composite Macrogels from Cellulose Fiber and Cellulose Nanofiber as Intestine Delivery Vehicles for Probiotics. Luan, Qian,Zhang, Hao,Bao, Yuping,Zheng, Mingming,Shi, Jie,Tang, Hu,Huang, Fenghong,Zhou, Weijie. 2018

[17]Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Song, J.,Xiao, K.,Ke, Y. L.,Jiao, L. F.,Hu, C. H.,Zou, X. T.,Diao, Q. Y.,Shi, B.. 2014

[18]Antioxidant activity of Lactobacillus plantarum strains isolated from traditional Chinese fermented foods. Yang, Zhennai,Li, Shengyu,Zhao, Yujuan,Zhang, Li,Zhang, Xue,Huang, Li,Li, Da,Niu, Chunhua,Yang, Zhennai,Zhang, Li,Yang, Zhennai,Wang, Qiang.

[19]Complete genome sequence of Lactobacillus helveticus MB2-1, a probiotic bacterium producing exopolysaccharides. Li, Wei,Chen, Xiaohong,Rui, Xin,Jiang, Mei,Zhang, Qiuqin,Dong, Mingsheng,Xia, Xiudong,Zhou, Jianzhong.

[20]Dietary supplementation of probiotic Bacillus PC465 isolated from the gut of Fenneropenaeus chinensis improves the health status and resistance of Litopenaeus vannamei against white spot syndrome virus. Chai, Peng-Cheng,Song, Xiao-Ling,Xu, Hua,Huang, Jie,Huang, Jie,Chen, Guo-Fu.

作者其他论文 更多>>

微信小程序