Growth performance, reproductive traits and offspring survivability of genetically modified rams overexpressing toll-like receptor 4

文献类型： 外文期刊

第一作者： Yao, Yu-Chang

作者： Yao, Yu-Chang;Song, Xu-Ting;Li, Wu;Han, Hong-Bing;Deng, Shou-Long;Liu, Guo-Shi;Lian, Zheng-Xing;Yao, Yu-Chang;Song, Xu-Ting;Liu, Yu-Feng;Qi, Mei-Yu;He, Hai-Juan;Lu, Ming-Hai;Zhang, Yun-Hai;Wang, Su-Mei

作者机构：

关键词： Toll-like receptor 4;Genetically modified sheep;Growth performance;Reproductive traits;Offspring survivability

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Genetic modification provides a means to enhancing disease resistance in animals. Toll-like receptor 4 (TLR4), a member of the TLR family, is critical for the recognition of lipopolysaccharide (LPS)/endotoxin from Gram-negative bacteria by host immune cells, which initiates cell activation and subsequently triggers a proinflammatory response to the invading pathogens. In this study, the first generation of genetically modified (GM) sheep overexpressing TLR4 was produced by microinjection for better disease resistance. Compared with wild-type (WT) rams, the GM rams have similar growth performance, basic semen quality and spermatozoon ultrastructure. The offspring birth rates after cervical artificial insemination were also similar between GM (90.32%) and WT (92.38%) rams. Overall, the presence and expression of the TLR4 transgene in the genome did not appear to interfere with normal semen production, reproductive traits and the ability of transgene transmission to offspring. The expression levels of TLR4, tumor necrosis factor and interferon gamma genes in monocyte/macrophages from GM sheep were significantly higher than that from WT sheep at early stages after LPS stimulation. The GM offspring born from the founder transgenic ram inseminated ewes had similar survival rate with WT offspring (88.89% vs 84.86%) at weaning. The TLR4 transgene showed no deleterious effects on growth performance, reproductive traits and offspring survivability of GM rams. Therefore, the GM sheep over expressing TLR4 provide a powerful experimental model for analyzing function of TLR4 in vivo during infection and inflammation. (C) 2017 Elsevier Inc. All rights reserved.

分类号： Q95

  • 相关文献

[1]Genome-wide analysis reveals artificial selection on coat colour and reproductive traits in Chinese domestic pigs. Wang, Chao,Wang, Hongyang,Zhang, Yu,Liu, Bang,Tang, Zhonglin,Li, Kui.

[2]The genetic diversity of perennial Leymus chinensis originating from China. Liu, Z. P.,Li, X. F.,Li, H. J.,Yang, Q. C.,Liu, G. S.. 2007

[3]Simultaneous identification and combined genotype effects analysis of pre-implantation protein 3 (prei3) and estrogen receptor (ESR) gene in pigs. Li, F. E.,Ye, L. Z.,Niu, B. Y.,Luo, L. F.,Shao, G. C.,Deng, C. Y.,Jiang, S. W.,Xiong, Y. Z.,Li, F. E.,Niu, B. Y.,Shao, G. C..

[4]Polymorphisms in the 5 '-flanking regions of the GH, PRL, and Pit-1 genes with Muscovy duck egg production. Zhang, D. X.,Xu, Z. Q.,Zhang, X. Q.,Zhang, D. X.,He, J.,Ji, C. L.,Zhang, Y..

[5]Haplotype analysis of TLR4 gene and its effects on milk somatic cell score in Chinese commercial cattle. Wang, Xing Ping,Zhuo Ma Luoreng,Guo, Dong Sheng,Li, Feng,Chen, Gang,Wang, Jin Ren,Gao, Shu Xin,Li, Jun Ya,Gao, Xue,Xu, Shang Zhong.

[6]Glycyrrhetinic acid extracted from Glycyrrhiza uralensis Fisch induces the expression of Toll-like receptor 4 in Ana-1 murine macrophages. Li, Li,Miao, Jian-Hua,Xu, Yong-Li,Peng, Li-Na,Qiu, Ya-Feng,Gao, Xiao-Qi,Shi, Zi-Xue,Shao, Dong-Hua,Wei, Jian-Chao,Ma, Zhi-Yong,Zhou, Ying. 2011

[7]The Structure and Sequence Analysis of TLR4 Gene in Cattle. Xu Shang-zhong,Gao Xue,Li Jun-ya,Ren Hong-yan,Chen Jin-bao,Wang Xing-ping,Luoreng Zhuo-ma. 2009

[8]Effects of dietary administering chitosan on growth performance, jejunal morphology, jejunal mucosal sIgA, occluding, claudin-1 and TLR4 expression in weaned piglets challenged by enterotoxigenic Escherichia coli. Xiao, Dingfu,Zhang, Bin,Xiao, Dingfu,Yin, Yulong,Feng, Zemeng,Tang, Zhiru,Hu, Xionggui,Wang, Jinquan. 2013

[9]chTLR4 pathway activation by Astragalus polysaccharide in bursa of Fabricius. Zhang Ruili,Yu Qun,Shi Guangliang,Liu Rui,Zhang Weiqian,Zhao Xia,Li Guangxing,Ge Ming,Yu Qun. 2017

[10]Polysaccharides from Acanthopanax senticosus enhances intestinal integrity through inhibiting TLR4/NF-B signaling pathways in lipopolysaccharide-challenged mice. Han, Jie,Liu, Lixia,Chen, Jing,Liu, Baoshan,Yang, Di,Shen, Guoshun,Yu, Ning.

[11]Effect of a mixture of steam-flaked corn and soybeans on health, growth, and selected blood metabolism of Holstein calves. Zhang, Y. Q.,Meng, Q. X.,Zhang, Y. Q.,He, D. Ch.. 2010

[12]Effects of different raising systems on growth performance, carcass, and meat quality of medium-growing chickens. Li, Ying,Luo, Chenglong,Wang, Jie,Guo, Fuyou,Li, Ying,Luo, Chenglong,Wang, Jie,Guo, Fuyou. 2017

[13]A study on dietary l-lysine requirement of juvenile yellow catfish Pelteobagrus fulvidraco. Cao, J. M.,Chen, Y.,Zhu, X.,Huang, Y. H.,Zhao, H. X.,Li, G. L.,Lan, H. B.,Chen, B.,Chen, Y.,Pan, Q.. 2012

[14]The Dietary L-Methionine Requirement of the Juvenile Yellow Catfish Pelteobagrus fulvidraco. Chen, Yan,Cao, Jun-Ming,Huang, Yan-Hua,Zhao, Hong-Xia,Chen, Bing,Zhu, Xuan,Lan, Han-Bing,Chen, Yan,Pan, Qing,Chen, Yan,Cao, Jun-Ming,Huang, Yan-Hua,Zhao, Hong-Xia,Chen, Bing,Zhu, Xuan,Lan, Han-Bing. 2014

[15]Effects of dietary hawthorn extract on growth performance, immune responses, growth- and immune-related genes expression of juvenile golden pompano (Trachinotus ovatus) and its susceptibility to Vibrio harveyi infection. Tan, Xiaohong,Huang, Zhong,Zhou, Chuanpeng,Lin, Heizhao,Tan, Lianjie,Xun, Pengwei,Huang, Qian,Tan, Xiaohong,Sun, Zhenzhu,Huang, Zhong,Lin, Heizhao. 2017

[16]Nutritive Value of Sorghum Dried Distillers Grains with Solubles and its Effect on Performance in Geese. Wang, Chao,Huang, Yong,Zhao, Xianzhi,Gao, Guangliang,Wang, Yangming,Peng, Xiangwei,Wang, Qigui,Xie, Ming. 2018

[17]Effects of outdoor access on growth performance, carcass composition, and meat characteristics of broiler chickens. Chen, X.,Jiang, W.,Xu, G. F.,Zhang, X. B.,Wang, X. Q.,Tan, H. Z.,Wei, S.. 2013

[18]Effect of methionine source and dietary crude protein level on growth performance, carcass traits and nutrient retention in chinese color-feathered chicks. Xi, P. B.,Yi, C. F.,Lin, Y. C.,Zheng, C. T.,Jiang, Z. Y.,Vazquez-Afion, M.,Song, C. L.,Knight, C. D.. 2007

[19]Effects of Dietary Arachidonic Acid Levels on Growth Performance, Whole-Body Proximate Composition, Digestive Enzyme Activities and Gut Morphology of Juvenile Golden Pompano Trachinotus ovatus.. Qi, Chang-Le,Lin, Hei-Zhao,Huang, Zhong,Zhou, Chuan-Peng,Wang, Yun,Wang, Jun,Niu, Jin,Tan, Xiao-Hong,Zhao, Shu-Yan,Qi, Chang-Le,Tan, Xiao-Hong,Zhao, Shu-Yan,Lin, Hei-Zhao,Huang, Zhong. 2016

[20]Effects of Alfalfa Meal on Growth Performance and Gastrointestinal Tract Development of Growing Ducks. Jiang, J. F.,Song, X. M.,Huang, X.,Zhou, W. D.,Wu, J. L.,Zhu, Z. G.,Zheng, H. C.,Jiang, Y. Q.. 2012

作者其他论文 更多>>

微信小程序