Ultrasensitive fluorometric glutathione assay based on a conformational switch of a G-quadruplex mediated by silver(I)

文献类型： 外文期刊

第一作者： Ji, Danyang

作者： Ji, Danyang;Meng, Hongmin;Ge, Jia;Zhang, Lin;Bai, Dongmei;Li, Jianjun;Qu, Lingbo;Li, Zhaohui;Wang, Hongqi;Li, Zhaohui

作者机构：

关键词： Methylmesoporphyrin IX;G-quadruplex;Fluorescence;Quenching;Stern-Volmer plot;Cell extracts

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The authors describe a silver(I) mediated fluorescent assay for glutathione (GSH). An allosteric oligonucleotide strand containing a G-rich sequence is used to produce a G-quadruplex, and N-methylmesoporphyrin IX (NMM) is chosen as the fluorescent probe. In the absence of Ag(I), the DNA strand is partially intramolecularly hybridized to form a hairpin structure wherein the G-rich sequence is partially caged. On addition of Ag(I), the hairpin is disrupted by forming C-Ag(I)-C base pairs. As a result, the G-rich sequence is released and folds into a G-quadruplex structure, which is able to bind NMM to generate strong fluorescence at 612 nm. However, in the presence of GSH, due to the strong binding ability between GSH and Ag(I), the C-Ag(I)-C structure is not formed. Hence, the DNA probe reverts back to its original structure and fluorescence is not increased. Based on these findings, a method was worked out that has a detection limit as low as 3.5 nM. Due to the inherent selectivity of the interaction between GSH and Ag(I), the method is highly selective over common potential interfering species. It was successfully applied to the fluorometric determination of GSH in cell extracts.

分类号： O65`O655

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