Effect of sodium butyrate on cell proliferation and cell cycle in porcine intestinal epithelial (IPEC-J2) cells

文献类型： 外文期刊

第一作者： Qiu, Yueqin

作者： Qiu, Yueqin;Ma, Xianyong;Yang, Xuefen;Wang, Li;Jiang, Zongyong

作者机构：

关键词： Sodium butyrate;IPEC-J2 cells;Cell proliferation;Cell cycle arrest;Apoptosis

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Conflicting results have been reported that butyrate in normal piglets leads either to an increase or to a decrease of jejunal villus length, implying a possible effect on the proliferation of enterocytes. No definitive study was found for the biological effects of butyrate in porcine jejunal epithelial cells. The present study used IPEC-J2 cells, a non-transformed jejunal epithelial line to evaluate the direct effects of sodium butyrate on cell proliferation, cell cycle regulation, and apoptosis. Low concentrations (0.5 and 1 mM) of butyrate had no effect on cell proliferation. However, at 5 and 10 mM, sodium butyrate significantly decreased cell viability, accompanied by reduced levels of p-mTOR and PCNA protein. Sodium butyrate, in a dose-dependent manner, induced cell cycle arrest in G0/G1 phase and reduced the numbers of cells in S phase. In addition, relative expression of p21, p27, and pro-apoptosis bak genes, and protein levels of p21Waf1/Cip1, p27Kip1, cyclinD3, CDK4, and Cleave-caspase3 were increased by higher concentrations of sodium butyrate (1, 5, 10 mM), and the levels of cyclinD1 and CDK6 were reduced by 5 and 10 mM butyrate. Butyrate increased the phosphorylated form of the signaling molecule p38 and phosphorylated JNK. In conclusion, the present in vitro study indicated that sodium butyrate inhibited the proliferation of IPEC-J2 cells by inducing cell cycle arrest in the G0/G1 phase of cell cycles and by increasing apoptosis at high concentrations.

分类号： Q95`Q2

  • 相关文献

[1]The comparative study among different fractions of muscadine grape 'Noble' pomace extracts regarding anti-oxidative activities, cell cycle arrest and apoptosis in breast cancer. Song, Shiren,Lu, Jiang,Luo, Jianming,Wei, Zheng,Zhang, Yali,Luo, Jianming,Wei, Zheng,Huang, Yu,Lu, Jiang. 2017

[2]Identification of pinostilbene as a major colonic metabolite of pterostilbene and its inhibitory effects on colon cancer cells. Sun, Yue,Qiu, Peiju,Zhang, Lijuan,Sun, Yue,Wu, Xian,Cai, Xiaokun,Song, Mingyue,Zheng, Jinkai,Pan, Che,Qiu, Peiju,Xiao, Hang,Zhou, Shuangde,Tang, Zhonghai,Xiao, Hang,Zheng, Jinkai.

[3]Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells. Gao, Chun-qi,Zhao, Yin-ling,Sui, Wei-guo,Yan, Hui-chao,Wang, Xiu-qi,Li, Hai-chang. 2015

[4]The citrus flavonoid nobiletin inhibits proliferation and induces apoptosis in human pancreatic cancer cells in vitro. Zhang, Yu,Li, Hongjun,He, Zhifei,Zhang, Yu,Dou, Huating,Wu, Houjiu. 2014

[5]Chlorpyrifos is estrogenic and alters embryonic hatching, cell proliferation and apoptosis in zebrafish. Yu, Kaimin,Li, Guochao,Feng, Weimin,Liu, Lili,Zhang, Jiayu,Wu, Wei,Xu, Lei,Yan, Yanchun.

[6]Dietary supplementation of sodium butyrate may benefit growth performance and intestinal function in juvenile grass carp (Ctenopharyngodon idellus). Liu, Mengmei,Guo, Wei,Wu, Fan,Qu, Qicai,Tan, Qingsong,Gong, Wangbao.

[7]Sodium butyrate-induced histone hyperacetylation up-regulating WT1 expression in porcine kidney fibroblasts. Tan, Wentao,Wang, Aibing,Chu, Meiran,Shi, Liuxin,Hou, Xiaoxiao,Tang, Bo,Zhang, Xueming,Li, Ziyi,Chen, Yue,An, Peipei.

[8]Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells. Wang, Jing,Lv, Xiao-Wen,Shi, Jie-Ping,Hu, Xiao-Song. 2007

[9]Histone acetylation and reactive oxygen species are involved in the preprophase arrest induced by sodium butyrate in maize roots. Zhang, Qi,Wang, Pu,Hou, Haoli,Zhang, Hao,Tan, Junjun,Huang, Yan,Li, Yingnan,Li, Lijia,Tan, Junjun,Wu, Jinping,Qiu, Zhengming.

[10]Effects of Mycobacterium tuberculosis ESAT6-CFP10 Protein on Cell Viability and Production of Nitric Oxide in Alveolar Macrophages. Xie, Xiaoli,Han, Meng,Zhang, Liang,Liu, Laixing,Gu, Zuye,Yang, Mei,Yang, Hongjun. 2016

[11]SMALL GRAIN 1, which encodes a mitogen-activated protein kinase kinase 4, influences grain size in rice. Duan, Penggen,Xu, Ran,Zhang, Baolan,Li, Yunhai,Duan, Penggen,Rao, Yuchun,Zeng, Dali,Yang, Yaolong,Dong, Guojun,Qian, Qian,Rao, Yuchun. 2014

[12]MiR-34b-5p Suppresses Melanoma Differentiation-Associated Gene 5 (MDA5) Signaling Pathway to Promote Avian Leukosis Virus Subgroup J (ALV-J)-Infected Cells Proliferaction and ALV-J Replication. Li, Zhenhui,Luo, Qingbin,Xu, Haiping,Zheng, Ming,Abdalla, Bahareldin Ali,Feng, Min,Cai, Bolin,Zhang, Xiaocui,Nie, Qinghua,Zhang, Xiquan,Li, Zhenhui,Luo, Qingbin,Xu, Haiping,Zheng, Ming,Abdalla, Bahareldin Ali,Feng, Min,Cai, Bolin,Zhang, Xiaocui,Nie, Qinghua,Zhang, Xiquan,Li, Zhenhui,Luo, Qingbin,Xu, Haiping,Zheng, Ming,Abdalla, Bahareldin Ali,Feng, Min,Cai, Bolin,Zhang, Xiaocui,Nie, Qinghua,Zhang, Xiquan. 2017

[13]The multi-herbal formula Chong-Myung-Tang improves spatial memory and increases cell genesis in the dentate gyrus of aged mice. Liu, Lei,Zhang, Mingwei,Zhang, Ruifen,Lee, Mira,Hou, Jingang,Sung, Chang-Keun,Wang, Zhen. 2014

[14]Circular RNAs are abundant and dynamically expressed during embryonic muscle development in chickens. Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan,Ouyang, Hongjia,Chen, Xiaolan,Wang, Zhijun,Yu, Jiao,Jia, Xinzheng,Li, Zhenhui,Luo, Wei,Abdalla, Bahareldin Ali,Jebessa, Endashaw,Nie, Qinghua,Zhang, Xiquan. 2018

[15]Proliferation and bone-related gene expression of osteoblasts grown on hydroxyapatite ceramics sintered at different temperature. Wang, CY,Duan, YR,Markovic, B,Barbara, J,Howlett, CR,Zhang, XD,Zreiqat, H. 2004

[16]Melatonin improves reprogramming efficiency and proliferation of bovine-induced pluripotent stem cells. Bai, Chunyu,Li, Xiangchen,Gao, Yuhua,Yuan, Ziao,Hu, Pengfei,Wang, Hui,Liu, Changqing,Guan, Weijun,Ma, Yuehui,Gao, Yuhua,Yuan, Ziao,Hu, Pengfei,Wang, Hui,Liu, Changqing. 2016

[17]Toxicarioside A, isolated from tropical Antiaris toxicaria, blocks endoglin/TGF-beta signaling in a bone marrow stromal cell line. Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Mei, Wen-Li,Dai, Hao-Fu,Zhou, Peng,Li, Yue-Nan,Huang, Feng-Ying,Guo, Jun-Li,Tan, Guang-Hong. 2012

[18]Immune response in guinea pigs vaccinated with DNA vaccine of foot-and-mouth disease virus O/China99. Guo, HC,Liu, ZX,Sun, SQ,Bao, HF,Chen, YL,Liu, XT,Xie, QG.

[19]Suppression of cell-cycle progression by Jun dimerization protein-2 (JDP2) involves downregulation of cyclin-A2. Huang, Y-C,Lee, C-H,Tsai, E-M,Yokoyama, K. K.,Pan, J.,Nakade, K.,Masuzaki, S.,Murata, T.,Yoshiki, A.,Obata, Y.,Yokoyama, K. K.,Pan, J.,Zhu, Z-W,Hasegawa, H.,Yamaguchi, N.,Lee, C-H,Yang, W-C,Yokoyama, K. K.. 2010

[20]Reticuloendotheliosis Virus Inhibits the Immune Response Acting on Lymphocytes from Peripheral Blood of Chicken. Bi, Yulin,Xu, Lu,Qiu, Lingling,Wang, Shasha,Zhang, Yani,Chen, Yang,Zhang, Yang,Xu, Qi,Chang, Guobin,Chen, Guohong,Liu, Xiangping. 2018

作者其他论文 更多>>

微信小程序