A novel nitrilase from Ralstonia eutropha H16 and its application to nicotinic acid production

文献类型： 外文期刊

第一作者： Fan, Haiyang

作者： Fan, Haiyang;Chen, Lifeng;Wang, Hualei;Ren, Yuhong;Wei, Dongzhi;Sun, Huihui

作者机构：

关键词： Nitrilase;Ralstonia eutropha;Nicotinic acid;Biotransformation

期刊名称：BIOPROCESS AND BIOSYSTEMS ENGINEERING （ 影响因子：3.21； 五年影响因子：2.97 ）

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年卷期：

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收录情况： SCI

摘要： A novel aliphatic nitrilase, REH16, was found in Ralstonia eutropha H16 and overexpressed in Escherichia coli BL21(DE3), and its enzymatic properties were studied. The temperature and pH optima were 37 A degrees C and 6.6, respectively, and the best thermostability of the nitrilase was observed at 25 A degrees C, which preserved 95% of activity after 120 h of incubation. REH16 has a broad hydrolytic activity toward aliphatic and heterocyclic nitriles and showed high tolerance of 3-cyanopyridine; this enzyme could hydrolyze as high as 100 mM 3-cyanopyridine completely. To improve the 3-cyanopyridine conversion efficiency in an aqueous reaction system, water-miscible organic solvents were tested, and ethanol (10% v/v) was chosen as the optimal co-solvent. Finally, under optimized conditions, using the fed-batch reaction mode, total of 1050 mM 3-cyanopyridine was hydrolyzed completely in 20.8 h with eight substrate feedings, yielding 129.2 g/L production of nicotinic acid and thus showing a potential for industrial application.

分类号： Q7

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