Molecular cloning and functional characterization of eleven subtypes of interferon-alpha in Amur tigers (Panthera tigris altaica)

文献类型： 外文期刊

第一作者： Zhao, Hongjing

作者： Zhao, Hongjing;Ma, Jian;Wang, Yu;Liu, Juanjuan;Shao, Yizhi;Li, Jinglun;Jiang, Guangshun;Xing, Mingwei;Ma, Jian

作者机构：

关键词： Panthera tigris altaica;Interferon;Subtype;Antiviral activities;Species protection

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）

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收录情况： SCI

摘要： Interferon has a broad-spectrum of antiviral effects and represents an ideal choice for the development of antiviral drugs. Nonetheless, information about alpha interferon (IFN-alpha) is vacant in Amur tiger (Panthera tigris altaica), an endangered species and indigenous to northeast Asia. Herein, 11 PtIFN-alpha s genes, which encoded proteins of 164-165 amino acids, were amplified. Afterwards, expression and purification were conducted in Escherichia coli. In physicochemical analysis, PtIFN-alpha s were shown to be highly sensitive to trypsin and remained stable despite changes in pH and temperature. In feline kidney cells (F81)/vesicular stomatitis virus (VSV)/canine distemper virus (CDV)/avian influenza virus (AIV) systems, PtIFN-alpha s were demonstrated to have distinct antiviral activities, some of them (PtIFN-alpha and PtIFN-alpha 9) inhibited viral transcription levels more effectively than the other subtypes including Felis catus IFN-alpha, an effective therapeutic agent used for viral infections clinically. Additionally, PtIFN-alpha and PtIFN-alpha 9 can up-regulate the transcription and expression of p53, a tumor suppressor factor, which could promote apoptosis of virus-infected cells. In conclusion, we cloned and expressed 11 subtypes of PtIFN-alpha for the first time. Furthermore, PtIFN-alpha and PtIFN-alpha 9 were likely to be more efficient against both chronic viral infections and neoplastic diseases that affect the Amur tiger population. It will be of significant importance for further studies to protect this endangered species. (C) 2017 Published by Elsevier Ltd.

分类号： R392

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