Identification of Reference Genes for Studying Herbicide Resistance Mechanisms in Japanese Foxtail (Alopecurus japonicus)

文献类型： 外文期刊

第一作者： Xu, Hongle

作者： Xu, Hongle;Li, Jun;Wu, Xibao;Wang, Lingyue;Dong, Liyao;Xu, Hongle;Wu, Renhai;Su, Wangcang

作者机构：

关键词： ACCase;BestKeeper;fenoxaprop-P-ethyl;gene expression;geNorm;NormFinder;qPCR

期刊名称：WEED SCIENCE （ 影响因子：2.713； 五年影响因子：2.868 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Herbicide-resistant weeds pose a considerable threat to agriculture, but their resistance mechanisms are poorly understood. Differential gene expression analysis of a weed subjected to herbicide treatment is a key step toward more mechanistic studies. Such an analysis, often involving quantitative real-time PCR (qPCR), requires suitable reference genes as internal controls. In this study, we identified optimal reference genes in the noxious weed, Japanese foxtail. This weed has evolved resistance to acetyl-coenzyme A carboxylase (ACCase) inhibitors. We analyzed the stability of eight commonly used candidate reference genes (glyceraldehyde-3-phosphate dehydrogenase [GAPDH]; ubiquitin [UBQ]; capsine phosphatase [CAP]; beta-tubulin [TUB]; eukaryotic initiation factor 4a [EIF4A]; elongation factor-1 alpha [EF1]; 18S ribosomal RNA [18S]; 25S ribosomal RNA [25S]) from root, stem, and leaf tissue of plants that were either resistant or sensitive to ACCase inhibitors, with or without herbicide stress, using qPCR. The results were further ranked and analyzed using geNorm, NormFinder, and BestKeeper software. These analyses identified EF1 and UBQ in roots, EF1, TUB, CAP, and 18S in stems, and EF1, GAPDH, and 18S in leaves as suitable references for qPCR normalization. We have identified a set of reference genes that can be used to study herbicide resistance mechanisms in Japanese foxtail.

分类号： S451

  • 相关文献

[1]Identification of reference genes for reverse transcription quantitative real-time PCR normalization in pepper (Capsicum annuum L.). Wan, Hongjian,Yuan, Wei,Ruan, Meiying,Ye, Qingjing,Wang, Rongqing,Li, Zhimiao,Zhou, Guozhi,Yao, Zhuping,Zhao, Jing,Liu, Shujun,Yang, Yuejian. 2011

[2]Selection and evaluation of reference genes for quantitative gene expression studies in cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae). Shakeel, Muhammad,Zhu, Xun,Kang, Tinghao,Wan, Hu,Li, Jianhong,Zhu, Xun. 2015

[3]Identification and testing of reference genes for Sesame gene expression analysis by quantitative real-time PCR. Wei, Libin,Miao, Hongmei,Zhao, Ruihong,Han, Xiuhua,Zhang, Tide,Zhang, Haiyang.

[4]Selection of reliable reference genes for gene expression studies in Rhododendron micranthum Turcz. Yi, Shanjun,Qian, Yongqiang,Han, Lei,Sun, Zhenyuan,Liu, Junxiang,Ju, Guansheng,Fan, Chengming.

[5]Validation of Reference Genes for Relative Quantitative Gene Expression Studies in Cassava (Manihot esculenta Crantz) by Using Quantitative Real-Time PCR. Hu, Meizhen,Wang, Wenquan,Hu, Meizhen,Xia, Zhiqiang,Zhou, Xincheng,Wang, Wenquan,Hu, Meizhen,Wang, Wenquan,Hu, Wenbin. 2016

[6]In-depth analysis of internal control genes for quantitative real-time PCR in Brassica oleracea var. botrytis. Sheng, X. G.,Zhao, Z. Q.,Yu, H. F.,Wang, J. S.,Gu, H. H.,Zheng, C. F.. 2016

[7]Cloning of acetyl CoA carboxylase cDNA and the effects of spirodiclofen on the expression of acetyl CoA carboxylase mRNA in Panonychus citri. Yu, Shijiang,Tian, Huixia,Li, Xiaojiao,Yue, Jiansu,Yu, Shijiang,Tian, Huixia,Yang, Juan,Ding, Lili,Chen, Fei,Li, Xiaojiao,Yue, Jiansu,Liu, Haoqiang,Ran, Chun,Chen, Fei.

[8]Mechanisms of Resistance to Pyroxsulam and ACCase Inhibitors in Japanese Foxtail (Alopecurus japonicus). Feng, Yujuan,Gao, Yuan,Dong, Liyao,Li, Jun,Zhang, Yong.

[9]Mechanism of Fenoxaprop-&ITP&IT-ethyl Resistance in Italian Ryegrass (&ITLolium&IT &ITperenne&IT ssp &ITmultiflorum&IT) from China. Zhang, Pei,Wu, Han,Gao, Yuan,Zhang, Wei,Dong, Liyao,Xu, Hongle.

[10]Isolation of an aryloxyphenoxy propanoate (AOPP) herbicide-degrading strain Rhodococcus ruber JPL-2 and the cloning of a novel carboxylesterase gene (feh). Liu Hongming,Lou Xu,Yang Fan,Chen Dingbin,Zhu Jianchun,Xu Jianhong,Li Shunpeng,Hong Qing,Xu Jianhong,Ge Zhaojian. 2015

[11]The role of cytochrome P450 monooxygenase in the different responses to fenoxaprop-P-ethyl in annual bluegrass (Poa annua L.) and short awned foxtail (Alopecurus aequalis Sobol.). Wang, Hongchun,Li, Jun,Dong, Liyao,Wang, Hongchun,Li, Jun,Dong, Liyao,Wang, Hongchun,Lou, Yuanlai,Lv, Bo. 2013

[12]Reference gene selection for quantitative real-time PCR normalization in different cherry genotypes, developmental stages and organs. Ye, Xia,Zhang, Fangming,Tao, Yonghuan,Song, Shangwei,Fang, Jinbao.

[13]Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols. Ma, Mingchao,Zhou, Jing,Guan, Dawei,Jiang, Xin,Li, Jun,Ma, Mingchao,Ongena, Marc,Liu, Wenzheng,Zhou, Jing,Zhao, Baisuo,Guan, Dawei,Jiang, Xin,Li, Jun,Wei, Dan. 2018

[14]Single and joint toxicity assessment of four currently used pesticides to zebrafish (Danio rerio) using traditional and molecular endpoints. Wang, Yanhua,Wu, Shenggan,Chen, Jine,Zhang, Changpeng,Xu, Zhenlan,Li, Gang,Cai, Leiming,Shen, Weifeng,Wang, Qiang. 2018

[15]Development of indirect immunofluorescence assay for TCID50 measurement of grass carp reovirus genotype II without cytopathic effect onto cells. Wang, Qing,Xie, Hualiang,Zeng, Weiwei,Liu, Chun,Wu, Jiexing,Wang, Yingying,Li, Yingying,Xie, Hualiang,Wang, Linchuan,Bergmann, Sven M.. 2018

[16]Variable responses of ammonia oxidizers across soil particle-size fractions affect nitrification in a long-term fertilizer experiment. Zhang, Qian,Liang, Guoqing,Zhou, Wei,Myrold, David D..

[17]High Oxygen Concentration Increases the Abundance and Activity of Bacterial Rather than Archaeal Nitrifiers in Rice Field Soil. Ke, Xiubin,Lu, Wei,Ke, Xiubin,Conrad, Ralf.

[18]Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community. Yi, Neng,Long, Xiao-hua,Shao, Hong-bo,Yan, Shao-hua,Yi, Neng,Gao, Yan,Zhang, Zhi-yong,Guo, Jun-yao,Zhang, Zhen-hua,Yan, Shao-hua,Shao, Hong-bo. 2014

[19]Characterization of physiological response and identification of associated genes under heat stress in rice seedlings. Jiang, Hua,Sun, Guo-Chang,Xue, Da-Wei,Zhang, Xiao-Qin,Qian, Qian,Hu, Jiang,Guo, Long-Biao,Zeng, Da-Li,Dong, Guo-Jun,Qian, Qian.

[20]Dynamic distribution and tissue tropism of nervous necrosis virus in juvenile pompano (Trachinotus ovatus) during early stages of infection. Su, Youlu,Xu, Haidong,Ma, Hongling,Feng, Juan,Wen, Weigeng,Guo, Zhixun.

作者其他论文 更多>>

微信小程序