Abnormal Somatic Embryo Reduction and Recycling in Grapevine Regeneration

文献类型： 外文期刊

第一作者： Ji, Wei

作者： Ji, Wei;Luo, Yaoxing;Guo, Rongrong;Li, Xuexue;Zhou, Qi;Ji, Wei;Wang, Yuejin;Ma, Xiaohe

作者机构：

关键词： Somatic embryogenesis;Abnormal;Vitis vinifera L.;Thompson seedless

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.169； 五年影响因子：4.038 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The purpose of the study was to increase plant conversion efficiency and reduce waste of the abnormal somatic embryos; factors influencing both the embryo morphology in germination and the embryogenic callus reinduction rate in secondary embryogenesis were studied in Vitis vinifera L. 'Thompson Seedless.' Embryos having mono-, di-, and multiple-normal cotyledons, trumpet-shaped, fascicular, and fused abnormal cotyledons were observed during germination. Normal embryos of one, two, and multiple cotyledons have a relatively higher conversion rate (48.89-64.44%) than abnormal ones (2.22-22.22%). Histomorphology analysis of these embryos showed that normal somatic embryos had a better developed vasculature and shoot meristem than abnormal individuals. A lower percentage of abnormal embryos formed when they germinated on Murashige and Skoog's medium (MS) and Woody Plant Medium (WPM) both of which contained 15 g/l sucrose and no plant growth regulators. Of all the abnormal embryos, only fused cotyledon embryos were suitable for recycling, and they were reinduced into the embryogenic callus. Cotyledons showed a higher reinduction rate (72.22%) than the other two parts: hypocotyls (4.44%) and roots (0%). In addition, recycled abnormal embryos produced many morphogenic types and this secondary embryogenesis could be used for embryo proliferation.

分类号： Q94

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