Development and characterization of simple sequence repeat (SSR) markers based on a full-length cDNA library of Napier Grass (Pennisetum purpureum Schum)
文献类型: 外文期刊
第一作者: Wang, Jia
作者: Wang, Jia;Hu, Zhongli;Diao, Ying;Chen, Zhitong;Huang, Yibing;Jin, Surong
作者机构:
关键词: Napier Grass;Transcriptome;De novo assembly;EST-SSRs;Genetic diversity
期刊名称:GENES & GENOMICS ( 影响因子:1.839; 五年影响因子:1.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Napier grass (Pennisetum purpureum Schum.) is an economically important species used as a fodder crop, reflecting its high biomass, perennial nature and pest resistance. In order to understand the genomic information and development of this species based on SSR markers, the transcriptome for Napier grass was obtained using next-generation sequencing. A total of 117,076 sequence contigs were obtained, with lengths of 201-10,652 bp. Among these contigs, 44,313 (37.85%) sequences were annotated against public protein databases (E-value < 10(-5)). In addition, the contigs generated from the transcript sequencing were also analysed for the presence of simple sequence repeats (SSRs). A total of 12,744 SSR motifs were identified with in these contigs and corresponding primer pairs were designed. Empirical validation of a cohort of 200 SSRs was performed, with 34% being polymorphic for 20 Napier grass accessions. The polymorphic index content values for each primer ranged from 0.105 to 0.749, with an average of 0.409. The development of genetic, genomic resources and EST-SSRs for Napier grass will contribute to novel gene discovery and the marker-assisted selective breeding of this species.
分类号: Q
- 相关文献
作者其他论文 更多>>
-
Preparation of a novel ATP liposome and its regulation of postharvest senescence in Agaricus bisporus
作者:Jiang, Xin;Han, Jiali;Feng, Lei;Wang, Jia;Wang, Xiangyou;Guo, Yanyin;Li, Ling;Chen, Cunkun;Chen, Cunkun;Kitazawa, Hiroaki
关键词:Agaricus bisporus; ATP; Egg yolk lecithin; Liposome; Preservation
-
KIF5B-mediated internalization of FMDV promotes virus infection
作者:Zhang, Wei;Yang, Fan;Yang, Yang;Cao, Weijun;Shao, Wenhua;Wang, Jiali;Huang, Mengyao;Chen, Zhitong;Zhao, Xiaoyi;Li, Weiwei;Zhu, Zixiang;Zheng, Haixue;Zhang, Wei;Yang, Fan;Yang, Yang;Cao, Weijun;Shao, Wenhua;Wang, Jiali;Huang, Mengyao;Chen, Zhitong;Zhao, Xiaoyi;Li, Weiwei;Zhu, Zixiang;Zheng, Haixue
关键词:FMDV; VP1 protein; KIF5B; Endosome; Clathrin
-
Identification of Trichderma harzianum in postharvest Agaricus bisporus and a novel control approach using eucalyptus essential oil emulsion
作者:Han, Jiali;Jiang, Xin;Feng, Lei;Wang, Jia;Wang, Xiangyou;Guo, Yanyin;Li, Ling;Zhou, Qingxin;Kitazawa, Hiroaki
关键词:Agaricus bisporus; Antifungal mechanisms; Eucalyptus essential oil; Postharvest disease; Trichoderma harzianum species complex
-
Intradermal vaccination with Porcilis® Begonia can clinically protect against fatal PRV challenge with the highly virulent ZJ01 field strain
作者:Zhang, Lei;Liu, Fei;Zhang, Lei;Liu, Bei-Bei;Li, Wen-Liang;Hao, Fei;Wang, Jia;Gan, Yuan;Yuan, Ting;Feng, Zhi-Xin;Shao, Guo-Qing;van den Born, Erwin;Segers, Ruud P. A. M.;Raes, Maurice;Di, Di;Zhang, Lei;Liu, Bei-Bei;Li, Wen-Liang;Hao, Fei;Wang, Jia;Gan, Yuan;Yuan, Ting;Feng, Zhi-Xin;Shao, Guo-Qing
关键词:Pseudorabies virus; Intradermal; Vaccination; Pigs; Challenge
-
Sustained release of a novel bilayer packaging film loaded with binary microemulsion of melatonin/pummelo essential oil and its regulation of postharvest energy metabolism in Agaricus bisporus
作者:Feng, Lei;Wang, Jia;Jiang, Xin;Han, Jiali;Li, Ling;Wang, Xiangyou;Guo, Yanyin;Zhang, Na;Chen, Cunkun;Zhang, Na;Chen, Cunkun;Kitazawa, Hiroaki
关键词:Active soft packaging; Binary microemulsion; Mushrooms; Postharvest aging; Energy metabolism
-
Gut Microbial Adaptation to Varied Altitudes and Temperatures in Tibetan Plateau Yaks
作者:Zhu, Yanbin;Cidan, Yangji;Wang, Hongzhuang;Basang, Wangdui;Zhu, Yanbin;Zhu, Yanbin;Wang, Jia;Li, Kun
关键词:Tibetan plateau yak; intestinal microbiome; ecological resilience; next-generation sequencing
-
Failure behavior of pressure compensating emitter under different operation pressures in drip irrigation systems
作者:Hou, Peng;Hou, Peng;Zhang, Kai;Hou, Shance;Li, Jingzhi;Sun, Zeqiang;Xiao, Yang;Li, Yunkai;Ma, Changjian;Li, Yan;Sun, Zeqiang;Ma, Changjian;Zhang, Kai;Hou, Shance;Li, Jingzhi;Xiao, Yang;Li, Yunkai;Wang, Jia
关键词:Emitter clogging; Flow channel; Diaphragm performance; Relative average discharge; Hydraulic performance