Cell lines derived from the squash bug, Anasa tristis (Coreidae: Hemiptera)

文献类型： 外文期刊

第一作者： Goodman, Cynthia L.

作者： Goodman, Cynthia L.;Ringbauer, Joseph A., Jr.;Lincoln, Tamra Reall;Stanley, David;Li, Yao-Fa

作者机构：

关键词： Insect cell line;Hemiptera;Squash bug;Anasa tristis;DAF-PCR

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura's medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15). We designated the two cell lines as BCIRL-AtE-CLG11 and BCIRL-AtE-CLG15. From the AtE-CLG15 line, we isolated two sub-lines, A and B. Of these, the most consistently replicating line was AtE-CLG15A. We determined the doubling time of this line (190 h) and its mean cell diameter (14.5 +/- 0.7 mu m). We characterized the AtE-CLG15A line using DAF-PCR. The BCIRL-AtE-CLG15A cell line is now available for researchers world-wide.

分类号： Q95

  • 相关文献

[1]Presence of a short repeat sequence in internal transcribed spacer (ITS) 1 of the rRNA gene of Sogatella furcifera (Hemiptera: Delphacidae) from geographically different populations in Asia. Matsumoto, Yukiko,Sato, Yuki,Noda, Hiroaki,Fu, Qiang,Matsumura, Masaya.

[2]Design and selection of trap color for capture of the tea leafhopper, Empoasca vitis, by orthogonal optimization. Bian, Lei,Sun, Xiao-Ling,Luo, Zong-Xiu,Zhang, Zheng-Qun,Chen, Zong-Mao.

[3]Effect of parasitism on flight behavior of the soybean aphid, Aphis glycines. Wu, Kongming,Wyckhuys, Kris A. G.,Heimpel, George E..

[4]Attractiveness of host volatiles combined with background visual cues to the tea leafhopper, Empoasca vitis. Cai, Xiao-Ming,Xu, Xiu-Xiu,Bian, Lei,Luo, Zong-Xiu,Xin, Zhao-Jun,Chen, Zong-Mao,Cai, Xiao-Ming.

[5]Association of different endosymbionts with the whitefly species Bemisia tabaci and Trialeurodes vaporariorum (Sternorrhyncha : Aleyrodidae). Tan, ZJ,Xie, BY,Xiao, QM,Yang, YH,Wan, FH,Huang, SW.

[6]Development and use of a monoclonal antibody to detect semi-digested proteins of the English grain aphid, Sitobion avenae, in the guts of ladybird beetle predators. Gao, Shu-jing,Zhou, Xiao-rong,Pang, Bao-ping,Gao, Shu-jing,van Loon, Joop J. A.,Zhao, Gui-qin.

[7]Effects of pattern and timing of high temperature exposure on reproduction of the rose grain aphid, Metopolophium dirhodum. Ma, CS,Hau, B,Poehling, HM. 2004

[8]Complete mitochondrial genome of Aphis gossypii Glover (Hemiptera: Aphididae). Shuai Zhang,Junyu Luo,Chunyi Wang,Limin Lv,Chunhua Li,Weili Jiang,Jinjie Cui,Lubna Bashir Rajput.

[9]A one-step, single tube, duplex PCR to detect predation by native predators on invasive Bemisia tabaci MEAM1 and Frankliniella occidentalis. Wu, Xia,Zhou, Zhi-Xiang,Meng, Xiang-Qin,Wan, Fang-Hao,Zhang, Gui-Fen,Wan, Fang-Hao. 2014

[10]Different effects of exogenous jasmonic acid on preference and performance of viruliferous Bemisia tabaci B and Q. Liu, Yong,Shi, Xiaobin,Pan, Huipeng,Xie, Wen,Wang, Shaoli,Wu, Qingjun,Chen, Gong,Tian, Lixia,Zhang, Youjun,Zhou, Xuguo. 2017

[11]A method for field assessment of plant injury elicited by the salivary proteins of Apolygus lucorum. Lu, Yanhui,Liang, Gemei. 2013

[12]Non-host plant essential oil volatiles with potential for a "push-pull' strategy to control the tea green leafhopper, Empoasca vitis. Zhang, Zhengqun,Chen, Zongmao,Zhang, Zhengqun.

[13]Sex pheromone of the alfalfa plant bug, Adelphocoris lineolatus. Zhang, Tao,Mei, Xiang-Dong,Zhang, Kaixin,Wu, Kong-Ming,Ning, Jun,Zhang, Tao,Li, Yao-Fa.

[14]Plant defense responses induced by Bemisia tabaci Middle EastAsia Minor 1 salivary components. Yan, Ying,Zhang, HaiJing,Yang, YiTing,Zhang, Yan,Guo, JianYang,Liu, WanXue,Wan, FangHao,Wan, FangHao.

[15]Differences in host selection and performance between B and Q putative species of Bemisia tabaci on three host plants. Jiao, Xiaoguo,Xie, Wen,Wang, Shaoli,Wu, Qingjun,Pan, Huipeng,Liu, Baiming,Zhang, Youjun,Jiao, Xiaoguo.

[16]EPG waveform characteristics of solenopsis mealybug stylet penetration on cotton. Huang, Fang,Zhang, Pengjun,Zhang, Jinming,Lu, Yaobin,Tjallingii, W. Freddy,Tjallingii, W. Freddy,Lin, Jintian. 2012

[17]Eight polymorphic microsatellite markers developed in the green leaf bug, Lygus lucorum Meyer-Dur (Hemiptera : Miridae). Liu, Yu-Di,Yang, Zhong-Cheng,Wu, Kong-Ming. 2007

[18]Defense against Pieris rapae in cabbage plants induced by Bemisia tabaci biotype B. Huang, Hong,Shan, Hong-Wei,Liu, Tong-Xian,Zhang, Shi-Ze,Huang, Hong,Shan, Hong-Wei,Liu, Tong-Xian,Zhang, Fan,Wan, Fang-Hao. 2013

[19]Effect of cold storage on the biological fitness of Encarsia sophia (Hymenoptera: Aphelinidae), a parasitoid of Bemisia tabaci (Hemiptera: Aleyrodidae). Yang, Nian-Wan,Wan, Fang-Hao,Kidane, Dawit.

[20]Volatiles from non-host aromatic plants repel tea green leafhopper Empoasca vitis. Zhang, Zhengqun,Luo, Zongxiu,Gao, Yu,Bian, Lei,Sun, Xiaoling,Chen, Zongmao,Zhang, Zhengqun.

作者其他论文 更多>>

微信小程序