Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer
文献类型: 外文期刊
第一作者: Tang, Shan
作者: Tang, Shan;Liang, Lu-Jun;Si, Yan-Yan;Gao, Shuai;Wang, Jia-Xing;Liu, Lei;Liang, Jun;Zheng, Ji-Shen;Mei, Ziqing
作者机构:
关键词: native chemical ligation;post-translational modifications;protein chemical synthesis;proteolysis;ubiquitin
期刊名称:ANGEWANDTE CHEMIE-INTERNATIONAL EDITION ( 影响因子:15.336; 五年影响因子:14.205 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Chemical ubiquitination is an effective approach for accessing structurally defined, atypical ubiquitin (Ub) chains that are difficult to prepare by other techniques. Herein, we describe a strategy that uses a readily accessible premade isopeptide-linked 76-mer (isoUb), which has an N-terminal Cys and a C-terminal hydrazide, as the key building block to assemble atypical Ub chains in a modular fashion. This method avoids the use of auxiliary-modified Lys and instead employs the canonical and therefore more robust Cys-based native chemical ligation technique. The efficiency and capacity of this isoUb-based strategy is exemplified by the cost-effective synthesis of several linkage- and length-defined atypical Ub chains, including K27-linked tetra-Ub and K11/K48-branched tri-, tetra-, penta-, and hexa-Ubs.
分类号: O69
- 相关文献
作者其他论文 更多>>
-
Probiotics encapsulated by calcium pectin/chitosan-calcium pectin/sodium alginate-pectin-whey through biofilm-based microencapsulation strategy and their preventive effects on ulcerative colitis
作者:Rao, Yu;Deng, Jia;Zhang, Chengyi;Song, Yunlong;Liu, Lei
关键词:Biofilm-based probiotics microencapsulation; Microencapsulate characteristics; Tolerance properties; Ulcerative colitis; Gut microbiota
-
Maize actin depolymerizing factor 1 ( ZmADF1 ) negatively regulates pollen development
作者:Lv, Guihua;Wu, Zhengxin;Li, Xiangnan;Wang, Tingzheng;Chen, Jianjian;Liu, Lei;Zhang, Yuanyuan;Li, Yunfeng;Zhang, Yahui;Ren, Wenchuang
关键词:Actin depolymerizing factor 1; Overexpression; Gene editing; Maize; Pollen development
-
Integrated Multi-Omics Analysis to Reveal the Molecular Mechanisms of Inflorescence Elongation in Medicago sativa
作者:Huang, Xiuzheng;Liu, Lei;Qiang, Xiaojing;Meng, Yuanfa;Li, Zhiyong;Huang, Fan
关键词:alfalfa; inflorescence elongation; multi-omics; functional genes
-
Rhein: A potent immunomodulator empowering largemouth bass against MSRV infection
作者:Zhang, Xu;Liu, Lei;Wang, Huan;Qiu, Tianxiu;Zhou, Yan;Shan, Lipeng;Wang, Zixuan;Hu, Yang;Chen, Jiong;Zhang, Xu;Liu, Lei;Wang, Huan;Qiu, Tianxiu;Zhou, Yan;Shan, Lipeng;Wang, Zixuan;Hu, Yang;Chen, Jiong;Xue, Mingyang;Liu, Guanglu
关键词:Rhein; Immunomodulator; Largemouth bass; Apoptosis; Micropterus salmoides rhabdovirus; Antiviral
-
Metabolomic and physiological analysis of alfalfa (Medicago sativa L.) in response to saline and alkaline stress
作者:Guo, Rui;Zhou, Zeyu;Cai, Run;Liu, Lei;Wang, Ruixin;Sun, Yugang;Wang, Dan;Guo, Changhong;Yan, Zhe
关键词:Saline stress; Alkaline stress; Untargeted metabolomics; Alfalfa; Plant resistance
-
Long-term ecological restoration increased plant diversity and soil total phosphorus content of the alpine flowing sand land in northwest Sichuan, China
作者:Jiang, Xue;Liu, Lei;Ma, Yunlong;Qu, Yanping;Zeng, Houyuan;Jian, Banghong;Jiang, Xue;Yang, Jingtian;Liu, Lei;Deng, Dongzhou;Chen, Dechao;He, Li;Guan, Lingliang
关键词:Flowing sandy land; Alpine region; Ecological restoration durations; Vegetation functional group composition; Soil characteristics
-
Modulation of pectin on intestinal barrier function via changes in microbial functional potential and bile acid metabolism
作者:Yin, Chang;Wen, Xiaobin;Dang, Guoqi;Zhong, Ruqing;Meng, Qingshi;Feng, Xiaohui;Liu, Lei;Wu, Shusong;Chen, Liang;Zhang, Hongfu;Wu, Shusong;He, Jianhua;Chen, Liang
关键词:pectin; ileal microbiota; bile acid; intestinal health.