Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer

文献类型： 外文期刊

第一作者： Tang, Shan

作者： Tang, Shan;Liang, Lu-Jun;Si, Yan-Yan;Gao, Shuai;Wang, Jia-Xing;Liu, Lei;Liang, Jun;Zheng, Ji-Shen;Mei, Ziqing

作者机构：

关键词： native chemical ligation;post-translational modifications;protein chemical synthesis;proteolysis;ubiquitin

期刊名称：ANGEWANDTE CHEMIE-INTERNATIONAL EDITION （ 影响因子：15.336； 五年影响因子：14.205 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Chemical ubiquitination is an effective approach for accessing structurally defined, atypical ubiquitin (Ub) chains that are difficult to prepare by other techniques. Herein, we describe a strategy that uses a readily accessible premade isopeptide-linked 76-mer (isoUb), which has an N-terminal Cys and a C-terminal hydrazide, as the key building block to assemble atypical Ub chains in a modular fashion. This method avoids the use of auxiliary-modified Lys and instead employs the canonical and therefore more robust Cys-based native chemical ligation technique. The efficiency and capacity of this isoUb-based strategy is exemplified by the cost-effective synthesis of several linkage- and length-defined atypical Ub chains, including K27-linked tetra-Ub and K11/K48-branched tri-, tetra-, penta-, and hexa-Ubs.

分类号： O69

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