Practical Chemical Synthesis of Atypical Ubiquitin Chains by Using an Isopeptide-Linked Ub Isomer

文献类型: 外文期刊

第一作者: Tang, Shan

作者: Tang, Shan;Liang, Lu-Jun;Si, Yan-Yan;Gao, Shuai;Wang, Jia-Xing;Liu, Lei;Liang, Jun;Zheng, Ji-Shen;Mei, Ziqing

作者机构:

关键词: native chemical ligation;post-translational modifications;protein chemical synthesis;proteolysis;ubiquitin

期刊名称:ANGEWANDTE CHEMIE-INTERNATIONAL EDITION ( 影响因子:15.336; 五年影响因子:14.205 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Chemical ubiquitination is an effective approach for accessing structurally defined, atypical ubiquitin (Ub) chains that are difficult to prepare by other techniques. Herein, we describe a strategy that uses a readily accessible premade isopeptide-linked 76-mer (isoUb), which has an N-terminal Cys and a C-terminal hydrazide, as the key building block to assemble atypical Ub chains in a modular fashion. This method avoids the use of auxiliary-modified Lys and instead employs the canonical and therefore more robust Cys-based native chemical ligation technique. The efficiency and capacity of this isoUb-based strategy is exemplified by the cost-effective synthesis of several linkage- and length-defined atypical Ub chains, including K27-linked tetra-Ub and K11/K48-branched tri-, tetra-, penta-, and hexa-Ubs.

分类号: O69

  • 相关文献

[1]UBIQUITIN-SPECIFIC PROTEASES function in plant development and stress responses. Zhou, Huapeng,Cai, Jingqing,Zhao, Jinfeng,Patil, Suyash B..

[2]Enrichment and analysis of rice seedling ubiquitin-related proteins using four UBA domains (GST-qUBAs). Meng, Qingshi,Rao, Liqun,Meng, Qingshi,Pan, Yinghong,Meng, Qingshi,Pan, Yinghong.

[3]Co-expression of Ubiquitin gene and capsid protein gene enhances the potency of DNA immunization of PCV2 in mice. Fu, Fang,Li, Xuesong,Lang, Yuekun,Li, Xi,Li, Xuesong,Yang, Yuju,Tong, Guangzhi,Li, Guoxin,Zhou, Yanjun,Li, Xi. 2011

[4]Cloning and bioinformatics analysis of an ubiquitin gene of the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae). Du, Yu-zhou,Qiang, Cheng-kui,Zhou, Bao-ya,Qin, Yue-hua,Qiang, Cheng-kui,Li, Chang-chun,Qi, Ren-de. 2011

[5]Role of the ubiquitin-proteasome pathway on proteolytic activity in postmortem proteolysis and tenderisation of sheep skeletal muscle. Liu, Yue,Du, Manting,Li, Xin,Chen, Li,Zhang, Dequan,Liu, Yue,Tian, Jianwen,Shen, Qingwu.

[6]Influence of oxidation on the susceptibility of purified desmin to degradation by mu-calpain, caspase-3 and-6. Chen, Qianqian,Huang, Jichao,Huang, Ming,Zhou, Guanghong,Huang, Feng. 2014

[7]Influence on Cheddar cheese proteolysis and sensory characteristics of non-starter strain Lactobacillus plantarum. Bi, Weiwei,Zhang, Bixian,Bi, Weiwei,Zhao, Guixing,Wang, Guangjin,Zhang, Bixian,Liu, Haofei,Li, Jinrong,Chen, Lei,Zhao, Guixing,Bi, Weiwei,Zhao, Guixing,Lu, Shuwen. 2017

[8]Optimized gene synthesis, expression and purification of active salivary plasminogen activator alpha 2 (DSPAot2) of Desmodus rotundus in Pichia pastoris. Wei, Zhaorong,Li, Gangqiang,Liu, Dehu,Wang, Yueju,Li, Xiaokun,Liu, Dehu.

[9]Members of the Arabidopsis-SKP1-like gene family xhibit a variety of expression patterns and may play diverse roles in Arabidopsis. Zhao, DZ,Ni, WM,Feng, BM,Han, TF,Petrasek, MG,Ma, H.

[10]Proteolysis and cathepsin activities in the processing of dry-cured duck. Wang, D. Y.,Xu, X. L.,Zhou, G. H.,Wang, P.,Wang, D. Y.,Zhang, M. H.,Bian, H.,Dong, H.,Xu, W. M.,Zhu, Y. Z.,Liu, F.,Geng, Z. M.. 2014

[11]DWARF14, A Receptor Covalently Linked with the Active Form of Strigolactones, Undergoes Strigolactone-Dependent Degradation in Rice. Hu, Qingliang,He, Yajun,Wang, Lei,Liu, Simiao,Meng, Xiangbing,Liu, Guifu,Jing, Yanhui,Chen, Mingjiang,Song, Xiaoguang,Jiang, Liang,Yu, Hong,Wang, Bing,Li, Jiayang,Hu, Qingliang,He, Yajun,Wang, Lei,Liu, Simiao,Meng, Xiangbing,Liu, Guifu,Jing, Yanhui,Chen, Mingjiang,Song, Xiaoguang,Jiang, Liang,Yu, Hong,Wang, Bing,Li, Jiayang,Hu, Qingliang,He, Yajun,Li, Jiayang,Wang, Lei,Jiang, Liang. 2017

[12]Proteolytic activation of Bacillus thuringiensis Vip3Aa protein by Spodoptera exigua midgut protease. Zhang, Ling,Xu, Lian,Li, Jie,Niu, Li -Yang,Chen, Qing-Xi,Pan, Zhi-Zhen,Liu, Bo,Chen, Zheng,Zhu, Yu-Jing. 2018

[13]The effect of calcium chloride injection on shear force and caspase activities in bovine longissimus muscles during postmortem conditioning. Cao, J.,Cao, J.,Khan, M. A.,Shao, J.,Zhou, G.,Yu, X.,Xiang, Y..

[14]Expression profile of cathepsin B in the fat body of Bombyx mori during metamorphosis. Roh, Jong Yul,Je, Yeon Ho,Lee, Kwang Sik,Kim, Bo Yeon,Choo, Young Moo,Sohn, Hung Dae,Jin, Byung Rae,Yoon, Hyung Joo,Kang, Pil Don,Woo, Soo Dong,Gui, Zhong Zheng.

[15]Study on the Change of Muscle Proteins During the Half-Dried Salt-Cured Silver Carp (Hypophthalmichthys molitrix) Processing. Gao, Ruichang,Yuan, Li,Su, Li,Huang, Xingyi,Yu, Gang,Li, Laihao.

[16]Proteolytic Activation of Bacillus thuringiensis Cry2Ab through a Belt-and-Braces Approach. Xu, Lian,Zhang, Jing,Chen, Qing-Xi,Pan, Zhi-Zhen,Liu, Bo,Zhu, Yu-Jing.

作者其他论文 更多>>