Development of NBS-related microsatellite (NRM) markers in hexaploid wheat

文献类型: 外文期刊

第一作者: Qiao, Linyi

作者: Qiao, Linyi;Qiao, Linyi;Zhang, Xiaojun;Li, Xin;Zheng, Jun;Chang, Zhijian;Zhang, Lei

作者机构:

关键词: Wheat;Disease resistance;NBS genes;NRM markers

期刊名称:EUPHYTICA ( 影响因子:1.895; 五年影响因子:2.181 )

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年卷期:

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收录情况: SCI

摘要: NBS (nucleotide binding site) genes, one type of the most important disease-resistance genes in the plant kingdom, are usually found clustered in genome. In this study, a total of 2288 full-length NBS protein-coding sequences were isolated from the wheat (Triticum aestivum L.) genome, and 903 TaNBSs of which were found expressed in wheat. Meanwhile, 2203 microsatellite loci were detected within 1061 scaffolds containing TaNBS. The distribution of these microsatellite loci across wheat homologous groups (HG) is 20% HG2, 16% HG7, 15% HG1, 15% HG6, 12% HG4, 12% HG5 and 10% HG3. We developed 1830 NBS-related microsatellite (NRM) markers for the microsatellite loci on TaNBS-scaffold sequences. Among them, 342 NRM markers were developed for HG2 with the largest number of microsatellite loci, and 69 out of these markers were anchored to the wheat genetic map using mapping population. Then, a total of 26 2AS-NRM markers, nine 2BL-NRM markers and nine 2DL-NRM markers were integrated into the genetic maps carrying Yr69, Pm51 and Pm43, respectively. Finally, candidate sequences, within the gene clusters where Yr5 and Sr21 located, were analyzed according to the genomic position information of TaNBS and NRM markers. These NRM markers have clear chromosome locations and are correlated with potential disease resistance sequences, which can be manipulated to mapping or adding linkage markers of disease-resistance genes or QTLs, especially for those in the NBS gene clusters.

分类号: S3

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