Transcriptomic analysis reveals differentially expressed genes and a unique apoptosis pathway in channel catfish ovary cells after infection with the channel catfish virus

文献类型： 外文期刊

第一作者： Dawar, Farman Ullah

作者： Dawar, Farman Ullah;Hu, Xianqin;Dong, Xingxing;Xiong, Yang;Mei, Jie;Lin, Li;Dawar, Farman Ullah;Zhao, Lijuan;Zhou, Meng;Liang, Rishen;Babu, V. Sarath;Lin, Li;Hu, Xianqin;Li, Jun;Li, Jun;Lin, Li;Lin, Li

作者机构：

关键词： Channel catfish;Channel catfish ovary cells;CCV;Transcriptome;DEGs;Apoptosis

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： AbstractThe channel catfish virus (CCV) can cause lethal hemorrhagic infection in juvenile channel catfish, thereby resulting in a huge economic loss to the fish industry. The genome of the CCV has been fully sequenced, and its prevalence is well documented. However, less is known about the molecular mechanisms and pathogenesis of the CCV. Herein, the channel catfish ovary cells (CCO) were infected with CCV and their transcriptomic sketches were analyzed using an RNA sequencing technique. In total, 72,686,438 clean reads were obtained from 73,231,128 sequence reads, which were further grouped into 747,168 contigs. These contigs were assembled into 49,119 unigenes, of which 20,912 and 18,333 unigenes were found in Nr and SwissProt databases and matched 15,911 and 14,625 distinctive proteins, respectively. From these, 3641 differentially expressed genes (DEGs), comprising 260 up-regulated and 3381 down-regulated genes, were found compared with the control (non-infected) cells. For verification, 16 DEGs were analyzed using qRT-PCR. The analysis of the DEGs and their related cellular signaling pathways revealed a substantial number of DEGs that were involved in the apoptosis pathway induced by CCV infection. The apoptosis pathways were further elucidated using standard apoptosis assays. The results showed that CCV could induce extrinsic apoptosis pathway (instead of a mitochondrial intrinsic apoptosis pathway) in CCO cells. This study helps our understanding of the pathogenesis of CCV and contributes to the prevention of CCV infection in channel catfish.Highlights?The transcriptomic sketches of CCO cells were analyzed after CCV infection.?Thousands of genes in CCO cells were involved against CCV infection.?The apoptosis caused by CCV infection in CCO cells was observed.?The CCV infection could induce extrinsic apoptosis pathway in CCO cells.]]>

分类号： S9

  • 相关文献

[1]Trancriptome profiles of Amur sturgeon spleen in response to Yersinia ruckeri infection. Li, Shaowu,Zhang, Ying,Cao, Yongsheng,Wang, Di,Liu, Hongbai,Lu, Tongyan,Li, Shaowu,Zhang, Ying,Lu, Tongyan. 2017

[2]Transcriptome Analysis of Cucumber Roots Reveals Key Cold-Resistance Genes Induced by AM Fungi. Ma, Jun,Sun, Chao,Bai, Longqiang,Dong, Rongrong,Yan, Yan,Yu, Xianchang,He, Chaoxing,Li, Yansu,Ma, Jun,Zou, Zhirong,Sun, Chao. 2018

[3]Transcriptomic analysis of Mandarin fish brain cells infected with infectious spleen and kidney necrosis virus with an emphasis on retinoic acid-inducible gene 1-like receptors and apoptosis pathways. Hu, Xianqin,Dong, Xingxing,Zhao, Lijuan,Lan, Jiangfeng,Ji, Wei,Lin, Li,Fu, Xiaozhe,Li, Ningqiu,Wu, Shuqin,Hu, Xianqin,Fu, Xiaozhe,Li, Ningqiu,Wu, Shuqin,Lin, Li,Hu, Xianqin,Zhou, Weidong,Ai, Taoshan,Lin, Li.

[4]Expression profiles of NODs in channel catfish (Ictalurus punctatus) after infection with Edwardsiella tarda, Aeromonas hydrophila, Streptococcus iniae and channel catfish hemorrhage reovirus. Li, Min,Wang, Qi-long,Lu, Yang,Chen, Song-lin,Sha, Zhen-xia,Li, Min,Li, Qi,Lu, Yang. 2012

[5]Identification and screening of effective protective antigens for channel catfish against Streptococcus iniae. Wang, Yajun,Wang, Erlong,He, Yang,Wang, Kaiyu,Yang, Qian,Wang, Jun,Geng, Yi,Ouyang, Ping,Lai, Weimin,Wang, Yajun,Wang, Kaiyu,Geng, Yi,Chen, Defang,Huang, Xiaoli. 2017

[6]Repeated handling compromises the immune suppression and improves the disease resistance in overwintering channel catfish (Ictalurus punctatus). Yang, Bingyuan,Hu, Huihua,Tu, Yongqin,Han, Dong,Zhu, Xiaoming,Jin, Junyan,Yang, Yunxia,Xie, Shouqi,Yang, Bingyuan,Hu, Huihua,Tu, Yongqin,Wang, Cuicui,Han, Dong.

[7]Transcriptome Sequencing Identified Genes and Gene Ontologies Associated with Early Freezing Tolerance in Maize. Li, Zhao,Hu, Guanghui,Liu, Xiangfeng,Zhou, Yao,Zhang, Qian,Yang, Deguang,Zhang, Zhiwu,Li, Zhao,Hu, Guanghui,Zhang, Xu,Yuan, Xiaohui,Zhang, Zhiwu,Hu, Guanghui,Wang, Tianyu,Yuan, Xiaohui. 2016

[8]Hepatic transcriptome analysis of juvenile GIFT tilapia (Oreochromis niloticus), fed diets supplemented with different concentrations of resveratrol. Zheng, Yao,Wu, Wei,Hu, Gengdong,Meng, Shunlong,Fan, Limin,Song, Chao,Qiu, Liping,Chen, Jiazhang,Zhao, Zhixiang,Chen, Jiazhang,Chen, Jiazhang. 2018

[9]Exploring differentially expressed genes associated with fertility instability of S-type cytoplasmic male-sterility in maize by RNA-seq. Su Ai-guo,Song Wei,Shi Zi,Zhao Yan-xin,Xing Jin-feng,Zhang Ru-yang,Li Chun-hui,Luo Mei-jie,Wang Ji-dong,Zhao Jiu-ran. 2017

[10]Dynamics in the resistant and susceptible peanut (Arachis hypogaea L.) root transcriptome on infection with the Ralstonia solanacearum. Chen, Yuning,Ren, Xiaoping,Zhou, Xiaojing,Huang, Li,Yan, Liying,Lei, Yong,Liao, Boshou,Huang, Shunmou,Wei, Wenhui,Jiang, Huifang,Huang, Jinyong. 2014

[11]Alteration of gene expression profile in the roots of wild diploid Arachis duranensis inoculated with Ralstonia solanacearum. Chen, Y. N.,Ren, X. P.,Zhou, X. J.,Huang, L.,Huang, J. Q.,Yan, L. Y.,Lei, Y.,Wei, W. H.,Jiang, H. F.,Qi, Y..

[12]RNA-seq analysis of Brachypodium distachyon responses to Barley stripe mosaic virus infection. Wang, Guoxin,Wang, Ling,Cui, Yu,Yu, Meihua,Dang, Chen,Wang, Hao,Jin, Xuejiao,Yan, Lijie,Li, Dawei,Liu, Zhiyong,Wang, Ling,Cui, Yu,Wu, Qiuhong,Liu, Zhiyong. 2017

[13]A collection of 10,096 indica rice full-length cDNAs reveals highly expressed sequence divergence between Oryza sativa indica and japonica subspecies. Liu, Xiaohui,Lu, Tingting,Yu, Shuliang,Li, Ying,Huang, Yuchen,Huang, Tao,Zhang, Lei,Zhu, Jingjie,Zhao, Qiang,Fan, Danlin,Mu, Jie,Shangguan, Yingying,Feng, Qi,Guan, Jianping,Ying, Kai,Zhang, Yu,Lin, Zhixin,Sun, Zongxiu,Qian, Qian,Lu, Yuping,Han, Bin.

[14]The Caspase Pathway of Linoelaidic Acid (9t, 12t-C18:2)-Induced Apoptosis in Human Umbilical Vein Endothelial Cells. Bin, Qiu,Rao, Huan,Hu, Jiang-Ning,Liu, Rong,Fan, Ya-Wei,Li, Jing,Deng, Ze-Yuan,Zhong, Xianfeng,Bin, Qiu,Du, Fang-Ling. 2013

[15]Detection of DNA ladder during cotyledon senescence in cotton. Q.E. XIE,I.D. LIU,S.X. YU,R.F. WANG,Z.X. FAN,Y.G. WANG,F.F. SHEN.

[16]Effects of species and cellular activity of oviductal epithelial cells on their dialogue with co-cultured mouse embryos. Tan, Xiu-Wen,Ma, Suo-Feng,Yu, Jian-Ning,Zhang, Xia,Lan, Guo-Cheng,Liu, Xin-Yong,Han, Zheng-Bin,Tan, Jing-He. 2007

[17]Oxidative Stress-Mediated Cytotoxicity of Cadmium in Chicken Splenic Lymphocytes. Li, Jin-Long,Tang, Zhao-Xin,Li, Jin-Long,Li, Shu,Xu, Shi-Wen,Li, Hui-Xin,Wang, Xiao-Long. 2010

[18]Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells. Gao, Chun-qi,Zhao, Yin-ling,Sui, Wei-guo,Yan, Hui-chao,Wang, Xiu-qi,Li, Hai-chang. 2015

[19]Facile fabrication of graphene oxide loaded with silver nanoparticles as antifungal materials. Cui, Jianghu,Yang, Yunhua,Zheng, Mingtao,Liu, Yingliang,Xiao, Yong,Lei, Bingfu,Chen, Wei. 2014

[20]Mitophagy promotes replication of oncolytic Newcastle disease virus by blocking intrinsic apoptosis in lung cancer cells. Meng, Gang,Xia, Mao,Wang, Diancheng,Chen, Aiping,Wang, Hongwei,Yu, Decai,Wei, Jiwu,Yu, Decai,Wei, Jiwu,Wang, Yongshan. 2014

作者其他论文 更多>>

微信小程序