Simulation guided intramolecular orthogonal reporters for dissecting cellular oxidative stress and response

文献类型： 外文期刊

第一作者： Pan, Yecan

作者： Pan, Yecan;Weng, Rui;Qiu, Jing;Wang, Xinlu;Liao, Guangqin;Qian, Yongzhong;Pan, Yecan;Zhang, Linghao;Qin, Zhaohui;Zhang, Lingpu;Su, Xin;Xiao, Haihua;Zhang, Lingpu;Xiao, Haihua

作者机构：

关键词： DNA nanotechnology; Simulation; Fluorescence imaging; Reactive oxygen species (ROS); Apurinic; apyrimidinic endonuclease

期刊名称：NANO TODAY （ 影响因子：18.962； 五年影响因子：20.24 ）

ISSN： 1748-0132

年卷期： 2022 年 46 卷

页码：

收录情况： SCI

摘要： Cellular oxidative stress from effects of reactive oxygen species (ROS) and its induced molecular response play an important role in the pathogenesis of various diseases and important biological processes. Nanostructure-based fluorescent probes can simultaneously map the localization and abundance of ROS and its induced molecules in living cells. However, proximity-induced fluorophore interaction leads to low sensitivity and poor specificity. Here, we engineered intramolecular Orthogonal Reporters (iOR) based on DNA nanostructure scaffolds. Fluorescence reporters in iOR were precisely located with appropriate dis-tance by simulation eliminating the undesired interaction of fluorophores. iOR functionalized with nuclear localization signal peptide is capable of imaging ROS and its related DNA repair enzyme (APE1) in the nucleus and cytoplasm with high sensitivity and specificity. iOR discloses the strong positive relation of ROS and APE1 as well as their synergistic regulation in living cells and tumor-bearing mice. This fact is first presented by direct fluorescence imaging. This work reported a new procedure for developing nanos-tructure-based molecular probes and would find broad applications in cellular biology and nanotechnology.(c) 2022 Published by Elsevier Ltd.

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