Comprehensive mRNA and microRNA analysis revealed the effect and response strategy of freshwater fish, grass carp (Ctenopharyngodon idella) under geosmin exposure

文献类型： 外文期刊

第一作者： Zhang, Jun-ming

作者： Zhang, Jun-ming;Han, Huan;Li, Kang;Jin, Xi-chen;Ji, Shuang;Liu, Li-ping;Zhang, Jun-ming;Han, Huan;Li, Yi-chao;Fu, Bing;Li, Kang;Jin, Xi-chen;Ji, Shuang;Liu, Li-ping;Li, Yi-chao;Yu, Er-meng;Fu, Bing;Kaneko, Gen

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关键词： Geosmin; Transcriptomic; MicroFtNA sequencing; Cytochrome P450; UDP-glucuronosyltransferase

期刊名称：ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY （ 影响因子：6.8； 五年影响因子：6.9 ）

ISSN： 0147-6513

年卷期： 2024 年 269 卷

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收录情况： SCI

摘要： Geosmin is an environmental pollutant that causes off-flavor in water and aquatic products. The high occurrence of geosmin contamination in aquatic systems and aquaculture raises public awareness, however, few studies have investigated the response pathways of geosmin stress on freshwater fish. In this research, grass carp were exposed to 50 mu g/L geosmin for 96 h, liver tissue was sequenced and validated using real-time qPCR. In total of 528 upregulated genes and 488 down-regulated genes were observed, includes cytochrome P450 and uridine diphosphate (UDP)-glucuronosyltransferase related genes. KEGG analysis showed that chemical carcinogenesis-DNA adducts, metabolism of xenobiotics by cytochrome P450, drug metabolism-cytochrome P450 pathway was enriched. Common genes from the target genes of microRNAs and differential expression genes are enriched in metabolism of xenobiotics cytochrome P450 pathway. Two miRNAs (dre-miR-146a and miR-212-3p) down regulated their target genes (LOC127510138 and adh5, respectively) which are enriched cytochrome P450 related pathway. The results present that geosmin is genetoxic to grass carp and indicate that cytochrome P450 system and UDP-glucuronosyltransferase play essential roles in biotransformation of geosmin. MicroRNAs regulate the biotransformation of geosmin by targeting specific genes, which contributes to the development of strategies to manage its negative impacts in both natural and artificial environments.

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