文献类型： 外文期刊
第一作者： Li, Shangyun
作者： Li, Shangyun;Chen, Zeli;Wang, Zhe;Gao, Like;Chu, Bifan;Yang, Zefen;Wen, Guosong;Zhao, Mingfu;Li, Shangyun;Chen, Zeli;Yang, Zefen;Wen, Guosong;Li, Shangyun;Chen, Zeli;Wang, Zhe;Gao, Like;Chu, Bifan;Yang, Zefen;Wen, Guosong;Zhao, Mingfu;Anane, Rex Frimpong;Duan, Chunfang;Yu, Daihong
作者机构：
关键词： Dregea volubilis; HTS; Closterovirus
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.7； 五年影响因子：2.4 ）
ISSN： 0304-8608
年卷期： 2023 年 168 卷 5 期
页码：
收录情况： SCI
摘要： The complete genome sequence of a putative novel closterovirus, tentatively named "Dregea volubilis closterovirus 1" (DvCV1, GenBank accession no. MZ779122), infecting Dregea volubilis in China was determined using high-throughput sequencing (HTS). The complete genome sequence of DvCV1 consists of 16,165 nucleotides (nt) and contains nine ORFs. The genome structure of DvCV1 is typical of members of the genus Closterovirus. Complete genome sequence analysis showed that DvCV1 shares 41.4-48.4% nucleotide sequence identity with other known closteroviruses. The putative RNA-dependent RNA polymerase (RdRp), heat shock protein 70-like protein (HSP70h), and coat protein (CP) of DvCV1 share 46.80-62.65%, 31.06-51.80%, and 28.34-37.37% amino acid sequence identity, respectively, with the RdRp, HSP70h and CP of other closteroviruses. Phylogenetic analysis based on HSP70h aa sequences placed DvCV1 alongside other members of the genus Closterovirus in the family Closteroviridae. These results suggest that DvCV1 is a new member of the genus Closterovirus. This is the first report of a closterovirus infecting D. volubilis.
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