Improvement of the Thermostability of Xylanase from Thermobacillus composti through Site-Directed Mutagenesis

文献类型: 外文期刊

第一作者: Tian, Yong-Sheng

作者: Tian, Yong-Sheng;Xu, Jing;Lei-Chen;Fu, Xiao-Yan;Peng, Ri-He;Yao, Quan-Hong;Tian, Yong-Sheng;Tian, Yong-Sheng

作者机构:

关键词: Xylanase thermostability;Thermobacillus composti;disulfide bridge;structure

期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )

ISSN: 1017-7825

年卷期: 2017 年 27 卷 10 期

页码:

收录情况: SCI

摘要: Thermostability is an important property of xylanase because high temperature is required for its applications, such as wood pulp bleaching, baking, and animal feedstuff processing. In this study, XynB from Thermobacillus composti, a moderately thermophilic gram-negative bacterium, was modified via site-directed mutagenesis (based on its 3D structure) to obtain thermostable xylanase, and the properties of this enzyme were analyzed. Results revealed that the half-life of xylanase at 65 degrees C increased from 10 to 50 min after a disulfide bridge was introduced between the a-helix and its adjacent beta-sheet at S98 and N145. Further mutation at the side of A153E named XynB-CE in the C-terminal of this a-helix enhanced the half-life of xylanase for 60 min at 65 degrees C. Therefore, the mutant may be utilized for industrial applications.

分类号:

  • 相关文献

[1]Enhancing the Thermal Resistance of a Novel Acidobacteria-Derived Phytase by Engineering of Disulfide Bridges. Miao, Renyun,Liu, Tianhai,Cao, Xuelian,Wu, Xiang,Xie, Liyuan,Huang, Zhongqian,Peng, Weihong,Gan, Bingcheng,Tan, Hao,Miao, Renyun,Liu, Tianhai,Cao, Xuelian,Wu, Xiang,Xie, Liyuan,Huang, Zhongqian,Peng, Weihong,Gan, Bingcheng.

[2]Improved thermal performance of Thermomyces lanuginosus GH11 xylanase by engineering of an N-terminal disulfide bridge. Wang, Yawei,Fu, Zheng,Xiong, Hairong,Wang, Yawei,Fu, Zheng,Huang, Huoqing,Yao, Bin,Zhang, Huashan,Turunen, Ossi.

[3]Structures of the Ozonolysis Products and Ozonolysis Pathway of Aflatoxin B-1 in Acetonitrile Solution. Diao, Enjie,Shan, Changpo,Hou, Hanxue,Dong, Haizhou,Wang, Shanshan,Li, Minghua. 2012

[4]Analyses of the NAC Transcription Factor Gene Family in Gossypium raimondii Ulbr.: Chromosomal Location, Structure, Phylogeny, and Expression Patterns. Haihong Shang ,Wei Li,Changsong Zou,Youlu Yuan. 2013

[5]Structure, Morphology, Thermostability and Irradiation-Mediated Degradation Fractions of Hemicellulose Treated with gamma-Irradiation. Wang, Liuyang,Zhou, Hua,Liu, Yun,Su, Xiaojun,Wang, Keqin,Wu, Xiaofeng,Chen, Liang,Liu, Yun,Xiong, Xingyao. 2016

[6]Effects of ultrasound assisted extraction on the physiochemical, structural and functional characteristics of duck liver protein isolate. Zou, Ye,Li, Pengpeng,Zhang, Muhan,Sun, Zhilan,Sun, Chong,Geng, Zhiming,Xu, Weimin,Wang, Daoying,Wang, Li,Cai, Panpan,Xu, Xinglian,Wang, Daoying. 2017

[7]Structure of mitochondrial DNA control region of Pholis fangi and its phylogenetic implication. Li Lin,Zhang Hui,Gao Tianxiang,Sun Dianrong. 2014

[8]A Study on the Structure and Properties of Pineapple Leaf Viscose Fiber. He, J-Y.,Zhuang, Z-K.,Huang, T.,Li, M-F.,Deng, G-R.,Lian, W-W.,Ou, Z-Q.,Qin, S-M.,Zhang, J.,He, J-Y.,Li, M-F.. 2013

[9]Physical, Chemical, and Rheological Properties of Rice Husks Treated by Composting Process. Wu, Guofeng,Qu, Ping,Sun, Enhui,Chang, Zhizhou,Xu, Yueding,Huang, Hongying. 2015

[10]Synthesis, Structure and Mechanism of a Lithium Complex with Hexanuclear Cage Structure. Wang Li,Xue Xiao-Yan,Huang Shu-Ping,Chen Xia,Chen Xia,Chang Gong-Min. 2011

[11]Composition, structure, and physicochemical properties of sweet potato starches isolated by sour liquid processing and centrifugation. Deng, Fu-Ming,Mu, Tai-Hua,Zhang, Miao,Abegunde, Oluwaseyi Kemi.

[12]Special structure of mitochondrial DNA control region and phylogenetic relationship among individuals of the black rockfish, Sebastes schlegelii. Zhang, Hui,Song, Na,Gao, Tianxiang,Zhang, Hui,Zhang, Xiumei,Song, Na,Gao, Tianxiang,Zhang, Hui,Zhang, Yan.

[13]Design of a Brassica rapa core collection for association mapping studies. Zhao, Jianjun,Del Carpio, Dunia Pino,Basnet, Ram Kumar,Zhang, Ningwen,Li, Fei,Bucher, Johan,Visser, Richard G. F.,Bonnema, Guusje,Zhao, Jianjun,Artemyeva, Anna,Gao, Jie,Li, Fei,Wang, Xiaowu.

[14]Insights into the structural and physicochemical properties of small granular starches from two hydrophyte duckweeds, Spirodela oligorrhiza and Lemna minor. Chen, Lei,Chen, Lei,Yu, Changjiang,Ma, Yubin,Xu, Hua,Wang, Shumin,Wang, Yu,Zhou, Gongke,Yu, Changjiang,Liu, Xingxun.

[15]Identification of sequence repetitions in immunoglobulin folds. Ji, Xiaofeng,Wang, Haiying,Hao, Jianhua,Zheng, Yuan,Wang, Wei,Sun, Mi.

[16]Structure and Thermal properties of RHA/NR composite. Zeng, Zongqiang,Liu, Hongchao,Yu, Heping,Peng, Zheng. 2012

[17]Structure and function of seed storage proteins in faba bean (Vicia faba L.). Liu, Yujiao,Wu, Xuexia,Hou, Wanwei,Li, Ping,Sha, Weichao,Tian, Yingying. 2017

[18]Structural characterization and immuno-enhancing activity of a highly branched water-soluble beta-glucan from the spores of Ganoderma lucidum. Wang, Yatao,Liu, Yanfang,Zhou, Shuai,Zhang, Zhong,Wu, Di,Yan, Mengqiu,Tang, Qingjiu,Zhang, Jingsong,Wang, Yatao,Yan, Mengqiu,Yu, Huazheng.

[19]Effect of laboratory milling on properties of starches isolated from different flour millstreams of hard and soft wheat. Yu, Jinglin,Wang, Shujun,Wang, Jingrong,Li, Caili,Xin, Quanwei,Huang, Wei,Wang, Shuo,Zhang, Yan,He, Zhonghu,He, Zhonghu.

[20]Comparison of Structural Modification of Sugarcane Bagasse Cellulose Regenerated from Three Imidazolium-Based Ionic Liquids. Cui, Lihong,Wei, Xiaoyi,Fu, Tiaokun,Chang, Gang,Feng, Qin,Tang, Bing. 2017

作者其他论文 更多>>