Comparison of extraction methods of total microbial DNA from freshwater

文献类型: 外文期刊

第一作者: Li, P.

作者: Li, P.;Yang, S. F.;Lv, B. B.;Zhao, K.;Lin, M. F.;Tang, X. M.;Li, P.;Yang, S. F.;Lv, B. B.;Zhao, K.;Lin, M. F.;Tang, X. M.;Zhou, S.;Song, X.

作者机构:

关键词: Extraction efficiency;Freshwater;Microbial diversity;Polymerase chain reaction;Total DNA

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 1 期

页码:

收录情况: SCI

摘要: The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugation method, filtration-membrane method, and modified filtration-membrane method, by evaluating the quantity and purity of DNA extracts obtained from water. DNA extraction was analyzed by agarose gel electrophoresis, ultraviolet-spectroscopy, restriction enzyme digestion, and polymerase chain reaction. The results showed that the modified filtration-membrane method was the most efficient for extracting microbial DNA from freshwater with high integrity and purity and is suitable for molecular applications.

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