Molecular cloning and functional characterization of dihydroflavonol-4-reductase gene from Calibrachoa hybrida

文献类型： 外文期刊

第一作者： Chu, Yunxia

作者： Chu, Yunxia;Pan, Junsong;Wu, Aizhong;Cai, Run;Chu, Yunxia;Wu, Aizhong;Chen, Hairong

作者机构：

关键词： Homologous gene;Asp type;Flower color;Petunia hybrida

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

ISSN： 0304-4238

年卷期： 2014 年 165 卷

页码：

收录情况： SCI

摘要： Dihydroflavonol-4-reductase (DFR) catalyzes the reduction of dihydroflavonols in the anthocyanin biosynthetic pathway. In this study, we obtained the full-length cDNA of DFR from Calibrachoa hybrida, CaDFR, through RACE-based technique. The cDNA of CaDFR is 1,351 bp with an open reading frame of 1, 152 bp, which encodes a putative protein of 383 amino acids. The CaDFR protein is predicted with 42.74 KD in molecular weight and a pI value of 5.83. Sequence alignment showed that CaDFR had close relationship with DFRs from other species of the Solanaceae family, and it belonged to the Asp-type. We constructed 35S::CaDFR overexpression vector and transformed it into three cultivars of Petunia hybrida using the Agrobacterium-mediated method. Multiple transformants were selected in the presence of 50 mg/l kanamycin antibiotic and further verified by polymerase chain reaction and Southern blotting analyses. We observed that the bud and flower color of transgenic petunia altered comparing with that of control plants. The DFR activities varied in most color-changed transgenic petunias. These results confirm the identity and function of CaDFR and further suggest that integration of the CaDFR can be used to change flower color of transgenic plants. (C) 2013 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Co-suppression in transgenic Petunia hybrida expressing chalcone synthase A (chsA). Li, Y,Xi, YW,Zhang, ZK,Huang, XQ. 2001

[2]Expression analysis of dihydroflavonol 4-reductase genes in Petunia hybrida. Chu, Y. X.,Chen, H. R.,Wu, A. Z.,Chu, Y. X.,Wu, A. Z.,Cai, R.,Pan, J. S.. 2015

[3]Identification of molecular markers associated with the double flower trait in Petunia hybrida. Liu, Caixian,He, Yanhong,Gou, Tianyun,Li, Xin,Ning, Guogui,Bao, Manzhu,Liu, Caixian,Li, Xin.

[4]Residents' preferences for roses, features of rose plantings and the relations between them in built-up areas of Beijing, China. Wang, Hua,Yang, Yuan,Li, Maofu,Liu, Jiashen,Jin, Wanmei,Wang, Hua,Yang, Yuan,Li, Maofu,Liu, Jiashen,Jin, Wanmei,Yang, Yuan. 2017

[5]Mapping Genes Governing Flower and Seedcoat Color in Asparagus Bean (Vigna unguiculata ssp sesquipedalis) Based on Single Nucleotide Polymorphism and Simple Sequence Repeat Markers. Xu, Pei,Hu, Tingting,Yang, Yuejian,Wu, Xiaohua,Wang, Baogen,Liu, Yonghua,Qin, Dehui,Lu, Zhongfu,Li, Guojing,Ehlers, Jeffrey,Close, Timothy. 2011

[6]Flavonoid content and expression analysis of flavonoid biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.) with double colors. Wu Yan-qing,Wei Meng-ran,Zhao Da-qiu,Tao Jun,Zhao Da-qiu,Tao Jun. 2016

[7]Flower pigment inheritance and anthocyanin characterization of hybrids from pink-flowered and white-flowered strawberry. Xue, Li,Zhang, Wei,Li, Yingxin,Wang, Jian,Lei, Jiajun,Wang, Zhigang.

作者其他论文 更多>>

微信小程序