Characterization of a Bifunctional O- and N-Glucosyltransferase from Vitis vinifera in Glucosylating Phenolic Compounds and 3,4-dichloroaniline in Pichia pastoris and Arabidopsis thaliana

文献类型： 外文期刊

第一作者： Xu, Zhi-Sheng

作者： Xu, Zhi-Sheng;Xue, Wei;Lin, Ya-Qiu;Xu, Jing;Zhu, Bo;Zhao, Wei;Peng, Ri-He;Yao, Quan-Hong;Xu, Zhi-Sheng;Xiong, Ai-Sheng;Lin, Ya-Qiu;Yao, Quan-Hong;Xue, Wei

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2013 年 8 卷 11 期

页码：

收录情况： SCI

摘要： 2,4,5-Trichlorophenol, 2,6-dimethylphenol, 3-methylcatechol, phenol, hydroquinone, catechol, and 3,4-dichloroaniline are present in the environment and are risky to humans and animals because of their wide applications in many industries. In this study, a putative uridine diphosphate glucose-dependent glycosyltransferase from Vitis vinifera (VvUGT72B1) displayed high O-glucosyltransferase or N-glucosyltransferase activity toward all these xenbiotics and was able to enhance the resistance of P. pastoris to them. Compared with wild-type Arabidopsis plants, VvUGT72B1-transgenic Arabidopsis plants showed higher resistance to all the xenobiotics except for phenol and exhibited higher removal efficiencies against all xenobiotics. Glucosides of 3-methylcatechol, 2,6-dimethylphenol, phenol, and 3,4-dichloroaniline were exported to the surrounding media by Arabidopsis plants while transgenic Arabidopsis plants exported more glucosides than wild-type Arabidopsis plants. Our findings have the potential to provide a broader spectrum remediation strategy for the phytoremoval and degradation of phenolic compounds and 3,4-dichloroaniline than previous works.

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