Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats

文献类型: 外文期刊

第一作者: Zhu, Yumin

作者: Zhu, Yumin;Yu, Xiaoming;Si, Fusheng;Yu, Ruisong;Dong, Shijuan;Li, Zhen;Zhu, Yumin;Yu, Ruisong;Dong, Shijuan;Li, Zhen;Yu, Xiaoming;Zhang, Yuanshu;Si, Fusheng;Ni, Yanyan;Huang, Yaowei

作者机构:

关键词: Hepatitis E virus (HEV);Infectious clone;Genotype 4;Rat animal model

期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )

ISSN: 0378-1135

年卷期: 2013 年 166 卷 3-4 期

页码:

收录情况: SCI

摘要: A cDNA clone of a genotype 4 swine hepatitis E virus (HEV) strain (SAAS-FX17), identified in Shanghai, has been constructed. Capped RNA transcripts were prepared in vitro and shown to be replication-competent in Huh7 cells. Sprague-Dawley (SD) rats administered the RNA transcripts by intrahepatic inoculation developed active infections as evidenced by fecal virus shedding and sero-conversion to anti-HEV. The former was first detected between 23 and 30 days post-inoculation (dpi) and persisted until 45 dpi. Sera of rats inoculated with RNA transcripts became anti-HEV positive between 30 and 40 dpi, and reverted to anti-HEV negative at 52 dpi. Our data indicate for the first time that intrahepatic inoculation of rats with RNA transcripts of an HEV cDNA clone may serve as an alternative animal model for HEV research. (C) 2013 Elsevier B.V. All rights reserved.

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