Methanol/sorbitol co-feeding induction enhanced porcine interferon-alpha production by P. pastoris associated with energy metabolism shift

文献类型: 外文期刊

第一作者: Gao, Min-Jie

作者: Gao, Min-Jie;Wu, Jian-Rong;Zheng, Zhi-Yong;Shi, Zhong-Ping;Zhan, Xiao-Bei;Lin, Chi-Chung;Li, Zhen;Yu, Rui-Song

作者机构:

关键词: Co-feeding;Energy metabolism;Induction;Pichia pastoris;Porcine interferon-alpha

期刊名称:BIOPROCESS AND BIOSYSTEMS ENGINEERING ( 影响因子:3.21; 五年影响因子:2.97 )

ISSN: 1615-7591

年卷期: 2012 年 35 卷 7 期

页码:

收录情况: SCI

摘要: The production of porcine interferon-alpha (pIFN-alpha) by Pichia pastoris was largely enhanced when adopting sorbitol/methanol co-feeding induction strategy at 30 A degrees C in a 10-L fermentor. Analysis of energy regeneration pattern and carbon metabolism revealed that major energy metabolism energizing pIFN-alpha synthesis shifted from formaldehyde dissimilatory energy metabolism pathway to TCA cycle under the methanol/sorbitol co-feeding induction strategy. The sorbitol/methanol co-feeding induction strategy weakened formaldehyde dissimilatory pathway and repressed the accumulation of toxic metabolite-formaldehyde, reduced theoretical oxygen consumption rate and oxygen supply requirement, and increased energy/methanol utilization efficiency so that more methanol could be effectively used for pIFN-alpha synthesis. As a result, pIFN-alpha antiviral activity reached a highest level of 1.8 x 10(7) IU/mL which was about 10- to 200-folds of those obtained under pure methanol induction at 20 and 30 A degrees C, respectively.

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