Characterization of a thermostable beta-glucuronidase from Thermotoga maritima expressed in Arabidopsis thaliana
文献类型: 外文期刊
第一作者: Xu, Jing
作者: Xu, Jing;Tian, Yong-Sheng;Peng, Ri-He;Zhu, Bo;Gao, Jian-Jie;Yao, Quan-Hong
作者机构:
关键词: Arabidopsis thaliana;beta-Glucuronidase;Thermostable enzyme;Thermotoga maritima
期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )
ISSN: 0175-7598
年卷期: 2012 年 95 卷 5 期
页码:
收录情况: SCI
摘要: TmGUSI, a gene identical to that encoding a thermostable beta-glucuronidase in the hyperthermophilic anaerobe Thermotoga maritima, has been synthesized using a PCR-based two-step DNA synthesis and codon optimization for plants, and expressed in both Escherichia coli and Arabidopsis thaliana. TmGUSI expressed in transformed E. coli cells exhibited maximum hydrolytic activity at 65 A degrees C and pH 6.5 and retained more than 80% activity after incubation at 85 A degrees C for 30 min. TmGUSI activity in transgenic A. thaliana plants containing TmGUSI was also stable over the temperature range 65-80 A degrees C. Our data suggest that beta-glucuronidase from T. maritima can serve as a useful thermostable marker in higher plants.
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