Screening of specific diagnostic peptides of swine hepatitis E virus

文献类型： 外文期刊

第一作者： Zhao, Kai

作者： Zhao, Kai;Zhu, Hong;Wu, Xiao;Tan, Furong;Wang, Jinbin;Tang, Xueming;Zhao, Kai;Yu, Ruisong;Li, Zhen;Zhu, Hong;Wu, Xiao;Tan, Furong;Wang, Jinbin;Tang, Xueming;Liu, Qiwen;Li, Jianyue;Yu, Ruisong;Li, Zhen

作者机构：

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN： 1743-422X

年卷期： 2009 年 6 卷

页码：

收录情况： SCI

摘要： Background: Swine hepatitis E virus (swHEV) is a zoonotic disease that is considered a major problem in pig production and presents a threat to human health. Elucidation of the major antigenic epitopes of swHEV is essential for the effective control of swHEV epidemics. Results: By bioinformatic analysis, we identified and then synthesized 12 peptides from open reading frames (ORFs) ORF1, ORF2 and ORF3, including swHEV-1 - swHEV-12. Using the results from ELISA, we selected swHEV-11 as the best candidate antigen and used it as a coating antigen for the development of peptide-based swine anti-HEV ELISA kits. The coefficient of variation (CV) the coefficient of variation (CV) varied between 4.3-7.2% in the same batch, and between 8.2-17.7% in six different batches. When comparing our swine peptide-based kit with the commercial recombinant-based kit, the humane anti-HEV IgG test had a 73.4% correspondence rate for them. Conclusion: This is the first systemic study to screen the diagnostic peptides of swHEV and our findings strongly suggest that peptide swHEV-11 is a potent diagnostic reagent of swHEV that could be used in the development of highly efficient diagnostic assays for the specific and highly sensitive detection of anti-HEV activity in swine serum samples.

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