Structural Analysis of a Bioactive Polysaccharide, PISP1, from the Medicinal Mushroom Phellinus igniarius

文献类型: 外文期刊

第一作者: Yang, Yan

作者: Yang, Yan;Zhang, JingSong;Liu, YanFang;Tang, QingJiu;Ye, LiBin

作者机构:

关键词: Phellinus igniarius;heteropolysaccharide;structural analysis;NMR spectroscopy;bioactivity

期刊名称:BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY ( 影响因子:2.043; 五年影响因子:2.017 )

ISSN: 0916-8451

年卷期: 2009 年 73 卷 1 期

页码:

收录情况: SCI

摘要: PISP1, a heteropolysaccharide isolated from fruiting bodies of Phellinus igniarius by hot aqueous extraction and purified by DEAE-Sepharose anion-exchange and gel filtration chromatography, is composed of fucose, galactose, mannose, and 3-O-Me-galactose in a ratio of 1:2:1:2. Methylation, monosaccharide analysis, and NMR studies (H-1 NMR, C-13 NMR, COSY-45 degrees, TOCSY, ROESY, HSQC, and HMBC) revealed that PISP1 had a backbone consisting of 1,6-disubstituted-3-O-Me-alpha-D-galactopyranosyl residue, 1,3,6-trisubstituted-alpha-D-manopyranosyl residue, 1,4-disubstituted-alpha-D-galactopyranosyl residue, and 1,2-disubstituted-alpha-D-galactopyranosyl residue, and had a 1-substituted-alpha-L-fucopyranosyl terminal attached to O-3 of a manopyranosyl residue. Preliminary bioactivity tests conducted in vitro revealed that PISP1 stimulated the proliferation of mouse spleen lymphocytes.

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