Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase
文献类型: 外文期刊
第一作者: Cao, MX
作者: Cao, MX;Huang, JQ;Yao, QH;Liu, SJ;Wang, CL;Wei, ZM
作者机构:
关键词: NITS;cell-permeable Cre recombinase;marker-free;transgenic rice plants;biosafety
期刊名称:MOLECULAR BIOTECHNOLOGY ( 影响因子:2.695; 五年影响因子:2.303 )
ISSN: 1073-6085
年卷期: 2006 年 32 卷 1 期
页码:
收录情况: SCI
摘要: The removal of selected marker genes from transgenic plants is necessary to address biosafety concerns and to carry out further experiments with transgenic organisms. In the present study, the 12-amino-acid membrane translocation sequence (MTS) from the Kaposi fibroblast growth factor (FGF)-4 was used as a carrier to deliver enzymatically active Cre proteins into living plant cells, and to produce a site-specific DNA excision in transgenic rice plants. The process, which made cells permeable to Cre recombinase-mediated DNA recombination, circumvented the need to express Cre under spatiotemporal control and was proved to be a simple and efficient system to achieve marker-free transgenic plants. The ultimate aim of the present study is to develop commercial rice cultivars free from selected marker genes to hasten public acceptance of transgenic crops.
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