Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase

文献类型： 外文期刊

第一作者： Cao, MX

作者： Cao, MX;Huang, JQ;Yao, QH;Liu, SJ;Wang, CL;Wei, ZM

作者机构：

关键词： NITS;cell-permeable Cre recombinase;marker-free;transgenic rice plants;biosafety

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN： 1073-6085

年卷期： 2006 年 32 卷 1 期

页码：

收录情况： SCI

摘要： The removal of selected marker genes from transgenic plants is necessary to address biosafety concerns and to carry out further experiments with transgenic organisms. In the present study, the 12-amino-acid membrane translocation sequence (MTS) from the Kaposi fibroblast growth factor (FGF)-4 was used as a carrier to deliver enzymatically active Cre proteins into living plant cells, and to produce a site-specific DNA excision in transgenic rice plants. The process, which made cells permeable to Cre recombinase-mediated DNA recombination, circumvented the need to express Cre under spatiotemporal control and was proved to be a simple and efficient system to achieve marker-free transgenic plants. The ultimate aim of the present study is to develop commercial rice cultivars free from selected marker genes to hasten public acceptance of transgenic crops.

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