The protective immune response induced by B cell epitope of classical swine fever virus glycoprotein E2

文献类型： 外文期刊

第一作者： Liu, SG

作者： Liu, SG;Tu, CC;Wang, CL;Yu, XL;Wu, JM;Guo, SP;Shao, ML;Gong, Q;Zhu, QH;Kong, XG

作者机构：

关键词： classical swine fever virus;envelope glycoprotein E2;epitope vaccine

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2006 年 134 卷 1-2 期

页码：

收录情况： SCI

摘要： Classical swine fever virus (CSFV) envelope glycoprotein E2 is a major protective immunogen responsible for eliciting neutralizing antibodies and conferring protective immunity against the virus. Based on the core sequence (TAVSPTTLR, 829-837 aa) of the B cell linear epitope of the CSFV E2 protein identified by Lin et al. [Lin, M., Lin, F., Mallory, M., Alfonso, C., 2004. Deletions of structural glycoprotein E2 of classical swine fever virus strain Alfort/187Resolve a lineal epitope of monoclonal antibody WH303 and the minimal N-terminal domain essential for binding immunoglobulin G antibodies of a pig hyperimmune serum. J. Virol. 74 (24), 11619-11625], two oligonucleotides MF and MR were synthesized and used to construct by PCR a gene cassette encoding a 15 amino acid polypeptide M (CTAVSPTTLRTEVVK), which spans 828-842 amino acids of E2. The gene cassette was fused in-frame to 3' terminal of glutathione S transferase gene (GST) of the prokaryotic expression vector pGEX-6p-1, resulting in the recombinant plasmid pGEX-M. After transformation into Escherichia coli BL21 a soluble fusion protein GST-M with expected size of 28 kDa was expressed after inducing with isopropyl-p-D-thiogalactoside (IPTG). Enzyme-linked immunosorbent assay (ELISA) and Western blot analysis showed that the purified GST-M had good reactivity with swine anti-CSFV serum and rabbit anti-CSFV E2 serum. Further vaccination trials showed that the fusion protein GST-M could elicit effectively immune response protecting rabbits and pigs from virulent challenge. This study showed a possibility for developing epitope-based vaccines against CSFV. (c) 2005 Elsevier B.V. All rights reserved.

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