Characterization of energy-transduction in thermal pretreated chloroplast from spinach

文献类型： 外文期刊

第一作者： Peng, JX

作者： Peng, JX;Wang, CZ;Zou, YL;Chen, GY;Qian, YQ;Yuan, Q;Wang, GQ

作者机构：

关键词： Spinacia oleracea;chloroplast;thermal pretreatment;energy-transduction;permeability of thylakoid membranes;coupling factor complex

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2001 年 43 卷 1 期

页码：

收录情况： SCI

摘要： Characterization of energy-transduction on die chloroplast thylakoid membranes from spinach (Spinacia oleracca L.) after thermal pretreatment was investigated. The related reactions of energy-transduction in chloroplasts were seriously affected by thermal pretreatment. The results were obtained as following: (1) The rate of cyclic photophosphorylation declined when the pretreatment temperature increased in the range of 25 to 45 degreesC. (2) The thermal pretreatment led to a decrease of the activity of thylakoid membrane-bounded ATPase. (3) Proton uptake of chloroplasts acid the fluorescence quenching of 9-aminoacridine (9-AA) in thylakoid membrane decreased after the thermal pretreatment, but addition of dicyclohexylcarbodiimide (DCCD) could partially restore the fluorescence quenching of 9-AA. (4) Both the rates of fast phase in electrochroism absorption change at 515 nm and the millisecond delayed light emission (ms-DLE) of chloroplast showed a progressive decrease upon raising the temperature of pretreatment. (5) Immunbloting analysis showed that the thermal pretreatment caused the changes of protein content and the electrophoresis mobility of thylakoid membrane-bound ATPase and its alpha -subunit. (6) If the temperature of pretreatment were higher than 33 degreesC, oxygen uptake of PS I -mediated in the samples was rapidly inhibited, but addition of sinapine into the reaction medium could partially restore the ability of oxygen uptake in the samples. These results are briefly discussed in relation to the change of permeability of thylakoid membranes, the dissociation of coupling factor complex as well as accumulation of the radicals in the thylakoid membranes after thermal pretreatment.

分类号：

  • 相关文献

[1]PERFORMANCE OF THERMAL PRETREATMENT FOR PATHOGEN INACTIVATION OF FECAL SLUDGE. Yin, Fubin,Dong, Hongmin,Li, Zifu,Wang, Dongling.

[2]Performance of thermal pretreatment and mesophilic fermentation system on pathogen inactivation and biogas production of faecal sludge: Initial laboratory results. Yin, Fubin,Dong, Hongmin,Li, Zifu,Wang, Dongling,Ohlsen, Thomas.

[3]Identification of RNA editing sites in cotton (Gossypium hirsutum) chloroplasts and editing events that affect secondary and three-dimensional protein structures. Y. Jiang,S.L. Fan,M.Z. Song,J.N. Yu,S.X. Yu. 2012

[4]Genetic diversity in Chinese sorghum landraces revealed by chloroplast simple sequence repeats. Li, Ruyu,Zhang, Chunqing,Li, Ruyu,Zhang, Han,Guan, Yanan,Yao, Fengxia,Song, Guoan,Wang, Jiancheng,Zhou, Xincheng.

[5]Cytoplasmic diversity of the cotton genus as revealed by chloroplast microsatellite markers. Li, Pengbo,Hua, Jinping,Li, Pengbo,Liu, Huimin,Li, Zhaohu,Hua, Jinping. 2014

[6]Stable Expression of Basic Fibroblast Growth Factor in Chloroplasts of Tobacco. Wang, Yun-Peng,Wei, Zheng-Yi,Zhong, Xiao-Fang,Lin, Chun-Jing,Zhang, Yu-Ying,Liu, Yan-Zhi,Xing, Shao-Chen,Cai, Yu-Hong,Ma, Jian,Zhang, Yu-Ying. 2016

[7]Loss-of-function mutation of rice SLAC7 decreases chloroplast stability and induces a photoprotection mechanism in rice. Fan, Xiaolei,Wu, Jiemin,Chen, Taiyu,Chen, Hao,Zhou, Fei,Lin, Yongjun,Fan, Xiaolei,Wu, Jiemin,Chen, Taiyu,Chen, Hao,Zhou, Fei,Lin, Yongjun,Tie, Weiwei. 2015

[8]Effects of plow pan on SPAD value and chloroplast ultrastructure in leaves of spring maize. Li, G.,Yang, F. T.,Jiang, X. L.,Chen, F. X.,Cao, T. H.,Wang, L. C.. 2013

[9]Experimental reconstruction of double-stranded break repair-mediated plastid DNA insertion into the tobacco nucleus. Wang, Dong,Gu, Jinbao,Wang, Zhen,Zhu, Jian-Kang,Wang, Dong,Gu, Jinbao,Wang, Zhen,Zhu, Jian-Kang,David, Rakesh,Searle, Iain R.,Timmis, Jeremy N.,Yang, Songtao,Zhu, Jian-Kang. 2018

[10]Ultrastructural observation on chloroplast of the thermo-sensitive green-yellow banded leaf mutant in rice (Oryza sativa ssp. indica). Wang, YZ,Liu, YS,Sun, JS,Xie, R. 1999

[11]The complex character of photosynthesis in cucumber fruit. Sui, Xiaolei,Shan, Nan,Ren, Huazhong,Zhang, Zhenxian,Hu, Liping,Zhang, Cankui,Yu, Changqing,Turgeon, Robert. 2017

[12]Young Leaf Chlorosis 1, a chloroplast-localized gene required for chlorophyll and lutein accumulation during early leaf development in rice. Zhou, Kunneng,Ren, Yulong,Lv, Jia,Wang, Yihua,Liu, Feng,Zhou, Feng,Zhao, Shaolu,Chen, Saihua,Peng, Cheng,Jiang, Ling,Wan, Jianmin,Zhang, Xin,Guo, Xiuping,Cheng, Zhijun,Wang, Jiulin,Wu, Fuqing,Wan, Jianmin.

[13]The complete chloroplast genome of Lilium taliense, an endangered species endemic to China. Zhang, Qian,Zhang, Mingfang,Chen, Xuqing,Yang, Fengping,Xue, Jing,Du, Yunpeng,Zhang, Xiuhai,Bi, Yu.

[14]Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts. He, Dong-Mei,Qian, Kai-Xian,Shen, Gui-Fang,Zhang, Zhi-Fang,Li, Yi-Nue,Su, Zhong-Liang,Shao, Hong-Bo.

[15]INTERACTIVE EFFECTS OF SALINITY AND PROLINE ON RICE AT THE ULTRASTRUCTURAL LEVEL. Sha, Han-Jing,Hu, Wen-Cheng,Jia, Yan,Liu, Hua-Long,Wang, Jing-Guo,Zou, De-Tang,Zhao, Hong-Wei,Chang, Hui-Lin. 2017

[16]Map-Based Cloning of zb7 Encoding an IPP and DMAPP Synthase in the MEP Pathway of Maize. Lu, Xiao-Min,Hu, Xiao-Jiao,Zhao, Yuan-Zeng,Song, Wei-Bin,Zhang, Mei,Chen, Zong-Liang,Chen, Wei,Dong, Yong-Bin,Lai, Jin-Sheng,Lu, Xiao-Min,Hu, Xiao-Jiao,Zhao, Yuan-Zeng,Song, Wei-Bin,Zhang, Mei,Chen, Zong-Liang,Chen, Wei,Dong, Yong-Bin,Lai, Jin-Sheng,Lu, Xiao-Min,Wang, Zhen-Hua,Zhao, Yuan-Zeng. 2012

[17]Effects of chilling stress on protein and related gene expression in chloroplasts of sugarcane. Li, Yang-Rui,Huang, Qiao-Ling,Huang, Xing,Sun, Fu,Sun, Bo,Yang, Li-Tao,Li, Yang-Rui.

[18]Construction of Rice Site-Specific Chloroplast Transformation Vector and Transient Expression of EGFP Gene in Dunaliella Salina. Han, Xiaoxia,Zuo, Jia,Xie, Lingling,He, Ronghua,Gao, Jing,Cao, Mengliang,Yuan, Longping,Cao, Mengliang,Chang, Lan,Cao, Mengliang.

[19]High expression of foot-and-mouth disease virus structural protein VP1 in tobacco chloroplasts. Li, Y,Sun, M,Liu, JX,Yang, ZQ,Zhang, ZF,Shen, GF. 2006

[20]Response of Chloroplast Ultrastructure of Ear Leaves of Spring Maize to Field Managements. Wang Ren,Sun Wen-tao,Xing Yue-hua,An Jing-wen. 2011

作者其他论文 更多>>

微信小程序