Use of isoquinoline alkaloids as markers for identification of honey and pollen from Macleaya cordata (Willd.) R. Br

文献类型： 外文期刊

第一作者： Zhao, Lingling

作者： Zhao, Lingling;Liang, Xinwen;Wu, Liming;Xue, Xiaofeng;Wu, Liming;Xue, Xiaofeng;Zhang, Zhongyin;Zhao, Lingling;Cao, Wei

作者机构：

关键词： Food analysis;Food composition;Macleaya cordata (Wild.) R. Br;Honey;Pollen;Alkaloid;QuEChERS;UHPLC-MS/MS

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.556； 五年影响因子：4.89 ）

ISSN： 0889-1575

年卷期： 2018 年 66 卷

页码：

收录情况： SCI

摘要： Macleaya cordata (Wild.) R. Br. (McRB) is utilized in traditional medicine and is mainly distributed in North America, Europe, Japan, and China. McRB honey known as "mad honey" produced by honey bees from the nectar of McRB contains isoquinoline alkaloids that give rise to potential consumer risk. In this study, a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction procedure followed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-MS/MS) was developed and optimized for the determination of seven isoquinoline alkaloids in McRB bee pollen and honey. The results revealed the presence of seven alkaloids in McRB bee pollen, four of which were also detected in McRB honey. Protopine and allocryptopine were the two predominant alkaloids, with concentrations of 0.17-0.66 mg/kg and 0.068-0.19 mg/kg in McRB honey and 1.25*10(boolean AND)3-3.07*10(boolean AND)3 mg/kg and 1.12*10(boolean AND)3-2.52*10(boolean AND)3 mg/kg in McRB bee pollen, respectively. None of the seven alkaloids were detected in commercial honey (n = 130) or pollen samples (n = 30). This study shows that protopine and allocryptopine could serve as potential markers of honey and pollen specifically from McRB.

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