Exploring formation of turanose in honey via stable isotope labelling and high-resolution mass spectrometry analysis

文献类型： 外文期刊

第一作者： Yuan, Yuzhe

作者： Yuan, Yuzhe;Wu, Liming;Xu, Haitao;Mu, Guodong;Xue, Xiaofeng;Yan, Sha

作者机构：

关键词： Honey; Turanose; 13C-labelling; alpha-Glucosidase; Intermolecular isomerisation

期刊名称：FOOD CHEMISTRY （ 影响因子：8.5； 五年影响因子：8.2 ）

ISSN： 0308-8146

年卷期： 2024 年 460 卷

页码：

收录情况： SCI

摘要： Turanose, an isomer of sucrose, naturally exists in honey. Previous study indicated that turanose content increased gradually in acacia honey as honeybees brewed honey in the hive. However, it is unclear how turanose is generated in honey. We hypothesised that turanose was produced by enzymes from honeybees and performed a series of simulation experiments to prove this hypothesis. We found turanose in honey was produced by honeybees processing sucrose. Furthermore, we determined that sugar composition of simulated nectar influenced the turanose concentration in honey: when sucrose concentration was below 5%, turanose was difficult to form, whereas high concentration of fructose and limited glucose were beneficial in producing turanose. Using 13C-labelled sucrose tests combined with proteomics analysis, we identified that alpha-glucosidase converted sucrose to turanose through an intermolecular isomerisation process. This study reveals the formation mechanism of turanose in honey and assists in the scientific control and improvement of honey quality.

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