Screening and Binding Analysis of Flavonoids with Alpha-Amylase Inhibitory Activity from Lotus Leaf

文献类型： 外文期刊

第一作者： Liao, Liping

作者： Liao, Liping;Chen, Jing;Liu, Liangliang;Xiao, Aiping

作者机构：

关键词： alpha-amylase;flavonoids;inhibitors;lotus leaf

期刊名称：JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY （ 影响因子：1.838； 五年影响因子：1.9 ）

ISSN： 0103-5053

年卷期： 2018 年 29 卷 3 期

页码：

收录情况： SCI

摘要： Lotus leaf is gaining growing popularity due to its various benefits and widely usage. In this paper, ten flavonoids in lotus leaf extract were analyzed by high performance liquid chromatography (HPLC). Centrifugal ultrafiltration combined liquid chromatography was used to screen alphaamylase inhibitors from ten flavonoids mixture and the binding degrees ranged from 2.34 to 94.1%. The alpha-amylase inhibition and the 1,1-diphenyl-2-picrylhydrazyl (DPPH) antioxidant activity of ten flavonoids were verified as well. Apigenin, kaempferol and isorhamnetin with relatively higher binding degrees showed higher inhibition on alpha-amylase as well. The interactions between these flavonoids and alpha-amylase were investigated by spectroscopic method. As a result, the fluorescence quenching could be considered as static quenching because the quenching rate constant (K-q) values were higher than 2.0 x 10(10) L mol(-1) s(-1). The binding constants (log(10) Ka) of ten flavonoids were in the range of 5.971 to 7.417 L mol(-1). The hydrogenation of C-2= C-3 double bond of apigenin and quercetin decreased the affinity for alpha-amylase (1.92-and 2.82-fold). The hydroxylation on 3 and 3' position decreased the affinity for alpha-amylase. Moreover, the glycosylation with different sugar moiety improved in varying degrees of affinity. The hydrogen bond force might be important in the binding between alpha-amylase and flavonoids.

分类号：

  • 相关文献

[1]Molecular Structure-Affinity Relationship of Flavonoids in Lotus Leaf (Nelumbo nucifera Gaertn.) on Binding to Human Serum Albumin and Bovine Serum Albumin by Spectroscopic Method. Tang, Xiaosheng,Tang, Xiaosheng,Tang, Xiaosheng,Tang, Ping,Liu, Liangliang,Tang, Ping.

[2]Study on the interaction between 3 flavonoid compounds and alpha-amylase by fluorescence spectroscopy and enzymatic kinetics. Li, Y.,Gao, F.,Gao, F.,Zhao, C.,Shan, F.,Bian, J..

[3]Zopolrestat as a Human GlyoxalaseI Inhibitor and Its Structural Basis. Zhai, Jing,Zhang, Hong,Zhang, Ligping,Zhao, Yining,Chen, Sangke,Chen, Yunyun,Li, Qing,Hu, Xiaopeng,Peng, Xinyu,Yuan, Minggui. 2013

[4]Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates. Wang, Jianqing,Zhang, Yan,Lin, Zhanglin,Chen, Yilu,Lin, Min.

[5]Sesquiterpenoids and lignans from the roots of Valeriana officinalis L.. Wang, Peng-Cheng,Ran, Xin-Hui,Chen, Rui,Luo, Huai-Rong,Liu, Yu-Qing,Hu, Jiang-Miao,Huang, Sheng-Zhuo,Jiang, He-Zhong,Chen, Zhong-Quan,Zhou, Jun,Zhao, You-Xing,Ma, Qing-Yun,Zhao, You-Xing,Wang, Peng-Cheng.

[6]Ability of resveratrol to inhibit advanced glycation end product formation and carbohydrate-hydrolyzing enzyme activity, and to conjugate methylglyoxal. Shen, Yixiao,Sheng, Zhanwu,Shen, Yixiao,Xu, Zhimin. 2017

[7]Screening, Gene Cloning, and Characterizations of an Acid-Stable alpha-Amylase. Liu, Xinyu,An, Yi,Cheng, Kun,Wang, Mingdao,Yang, Sen,Chen, Hongge,Jia, Wei.

[8]Isolation of -Amylase Inhibitors from Kadsura longipedunculata Using a High-Speed Counter-Current Chromatography Target Guided by Centrifugal Ultrafiltration with LC-MS. Cen, Yin,Chen, Xiaoqing,Xiao, Aiping,Liu, Liangliang.

[9]Improving the Catalytic Performance of a Talaromyces leycettanus alpha-Amylase by Changing the Linker Length. Zhang, Duoduo,Luo, Xuegang,Zhang, Tongcun,Zhang, Duoduo,Luo, Xuegang,Zhang, Tongcun,Tu, Tao,Wang, Yuan,Li, Yeqing,Zheng, Fei,Wang, Xiaoyu,Bai, Yingguo,Huang, Huoqing,Su, Xiaoyun,Yao, Bin,Luo, Huiying.

[10]Seed micromorphology and germination characteristics of wild and cultivated pepper strains. Zhang Zhuqing,Dai Xiongze,Zou Xuexiao,Ou Lijun,Ou Lijun.

[11]Phytosterols in banana (Musa spp.) flower inhibit alpha-glucosidase and alpha-amylase hydrolysations and glycation reaction. Sheng, Zhanwu,Pan, Siyi,Sheng, Zhanwu,Ai, Binling,Zheng, Lili,Zheng, Xiaoyan,Dai, Haofu,Prinyawiwatkul, Witoon,Xu, Zhimin.

[12]Physicochemical characterization of enzymatically hydrolyzed heat treated granular starches. Mu, Tai-Hua,Abegunde, Oluwaseyi K.,Sun, Hong-Nan,Deng, Fu-Ming,Zhang, Miao.

[13]Overexpression of celB gene coding for beta-glucosidase from Pyrococcus furiosus using a baculovirus expression vector system in silkworm, Bombyx mori. Lin, Xu'Ai,Zhang, Wei,Chen, Yin,Bin Yao,Zhang, Zhi Fang.

[14]Analysis of alpha-amylase inhibitor from corni fructus by coupling magnetic cross-linked enzyme aggregates of alpha-amylase with HPLC-MS. Cen, Yin,Liu, Fang,Yu, Jingang,Jiang, Xinyu,Chen, Xiaoqing,Liu, Liangliang.

[15]Screening and separation of alpha-amylase inhibitors from Solanum nigrum with amylase-functionalized magnetic graphene oxide combined with high-speed counter-current chromatography. Cen, Yin,Chen, Xiaoqing,Xiao, Aiping,Liu, Liangliang. 2017

[16]Germplasm improvement for preharvest sprouting resistance in Chinese white-grained wheat: An overview of the current strategy. Xiao, SH,Zhang, XY,Yan, CS,Lin, H. 2002

[17]Preparation and Characterization of Nano Fe3O4/Carboxylation Chitosan/Alginate Sodium Magnetofluid by One Pot Method. Jiang, Jianfang,He, Chunyang,Xiong, Jun,Tu, Wenya,Jiang, Hao. 2016

[18]Effect of salinity on the rearing performance of juvenile golden pompano Trachinotus ovatus (Linnaeus 1758). Ma, Zhenhua,Guo, Huayang,Zheng, Panlong,Wang, Long,Jiang, Shigui,Zhang, Dianchang,Ma, Zhenhua,Ma, Zhenhua,Qin, Jian G..

[19]Constitutive expression of barley alpha-amylase in Pichia pastoris by high-density cell culture. Liu, Z. W.,Yin, H. X.,Yi, X. P.,Zhang, A. L.,Zhang, Z. H.,Shen, J. C.,Chen, L. P.,Luo, J. X.,Zhang, T. Y.,Luo, J. X.,Zhang, T. Y.,Fu, C. Y..

[20]Optimization of water-soluble polysaccharides from stem lettuce by response surface methodology and study on its characterization and bioactivities. Nie, Chenzhipeng,Zhu, Peilei,Wang, Mingchun,Ma, Shuping,Nie, Chenzhipeng,Zhu, Peilei,Wang, Mingchun,Ma, Shuping,Zhu, Peilei,Wang, Mingchun,Wei, Zihao.

作者其他论文 更多>>

微信小程序