A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway

文献类型： 外文期刊

第一作者： Zhang, Xiao-Bo

作者： Zhang, Xiao-Bo;Feng, Bao-Hua;Wang, Hui-Mei;Xu, Xia;Shi, Yong-Feng;He, Yan;Chen, Zheng;Sathe, Atul Prakash;Shi, Lei;Wu, Jian-Li

作者机构： China Natl Rice Res Inst, State Key Lab Rice Biol, Chinese Natl Ctr Rice Improvement, Hangzhou 310006, Zhejiang, Peoples R China;China Natl Rice Res Inst, State Key Lab Rice Biol, Chinese Natl Ctr Rice Improvement, Hangzhou 310006, Zhejiang, P

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 2020影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2018 年 60 卷 2 期

页码：

收录情况： SCI

摘要： We previously reported a spotted-leaf mutant pelota (originally termed HM47) in rice displaying arrested growth and enhanced resistance to multiple races of Xanthomonas oryzae pv. oryzae. Here, we report the map-based cloning of the causal gene OsPELOTA (originally termed spl(HM47)). We identified a single base substitution from T to A at position 556 in the coding sequence of OsPELOTA, effectively mutating phenylalanine to isoleucine at position 186 in the translated protein sequence. Both functional complementation and over-expression could rescue the spotted-leaf phenotype. OsPELOTA, a paralogue to eukaryotic release factor 1 (eRF1), shows high sequence similarity to Drosophila Pelota and also localizes to the endoplasmic reticulum and plasma membrane. OsPELOTA is constitutively expressed in roots, leaves, sheaths, stems, and panicles. Elevated levels of salicylic acid and decreased level of jasmonate were detected in the pelota mutant. RNA-seq analysis confirmed that genes responding to salicylic acid were upregulated in the mutant. Our results indicate that the rice PELOTA protein is involved in bacterial leaf blight resistance by activating the salicylic acid metabolic pathway.

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