Multi-mycotoxins analysis in raw milk by ultra high performance liquid chromatography coupled to quadrupole orbitrap mass spectrometry

文献类型： 外文期刊

第一作者： Zheng, Nan

作者： Zheng, Nan;Wen, Fang;Wang, Jiaqi;Mao, Jianfei;Zheng, Nan;Wen, Fang;Wang, Jiaqi;Mao, Jianfei;Zheng, Nan;Wen, Fang;Wang, Jiaqi;Mao, Jianfei;Guo, Lingan;Fu, Chengping;Ouyang, Huaxue;Zhong, Lingli;Lei, Shaorong;Mao, Jianfei;Guo, Lingan;Fu, Chengping;Ouyang, Huaxue;Zhong, Lingli;Lei, Shaorong

作者机构：

关键词： Ultra high performance liquid chromatography;Quadrupole orbitrap mass spectrometry;UHPLC/Q-Orbitrap;Milk;Mycotoxins;Immunoaffinity column

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2018 年 84 卷

页码：

收录情况： SCI

摘要： This paper presents the development and application of a sensitive and specific method for the analysis of multi-mycotoxins in raw milk using ultrahigh performance liquid chromatography quadrupole orbitrap mass spectrometry (UHPLC/Q-Orbitrap). Mycotoxins (a total of 14) included in this study were aflatoxin B-1, aflatoxin B-2, aflatoxin G(1), aflatoxin G(2), aflatoxin M-1, aflatoxin M-2, ochratoxin A, ochratoxin B, zearalenone, zearalanone, alpha-zeralanol, beta-zeralanol, alpha-zeralenol, and beta-zeralenol. Mycotoxins in milk were extracted and purified with a multi-mycotoxins AOZ immunoaffinity column in a single run, which made the matrix effect be negligible. The deliberate addition of small amount of acetonitrile was found to be beneficial for the extraction of mycotoxins from the complex milk matrix, especially for zearalenone and its derivatives. The final reconstituted extracts were analyzed using ultra high performance liquid chromatography coupled to quadrupole orbitrap mass spectrometry (UHPLC Q-Orbitrap mass spectrometry). The extraction recoveries are in the range of 60-106%, with coefficient of variation <15%. The limits of detection for the analytes are in the range of 0.0003-0.008 mu g/kg. Compared with a triple quadrupole mass method in MRM mode, the Q-orbitrap analyzer provides accurate masses of both the precursor and product ions, thus offers a higher level of confidence in analyte identification without the sacrifice of analytical performance. The method was successfully applied to investigate the occurrence of the 14 mycotoxins in a total of two hundred and fifty raw milk samples collected from 5 different provinces of China. (C) 2017 Published by Elsevier Ltd.

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