Modulation of Intestinal Epithelial Permeability in Differentiated Caco-2 Cells Exposed to Aflatoxin M1 and Ochratoxin A Individually or Collectively

文献类型： 外文期刊

第一作者： Gao, Yanan

作者： Gao, Yanan;Li, Songli;Wang, Jiaqi;Luo, Chaochao;Zhao, Shengguo;Zheng, Nan;Gao, Yanan;Li, Songli;Wang, Jiaqi;Luo, Chaochao;Zhao, Shengguo;Zheng, Nan;Gao, Yanan;Li, Songli;Wang, Jiaqi;Luo, Chaochao;Zhao, Shengguo;Zheng, Nan

作者机构：

关键词： aflatoxin M1;ochratoxin A;intestinal epithelial cells;tight junction;permeability

期刊名称：TOXINS （ 影响因子：4.546； 五年影响因子：4.8 ）

ISSN： 2072-6651

年卷期： 2018 年 10 卷 1 期

页码：

收录情况： SCI

摘要： Aflatoxin M1 (AFM1) and ochratoxin A (OTA) are mycotoxins commonly found in milk; however, their effects on intestinal epithelial cells have not been reported. In the present study, we show that AFM1 (0.12 and 12 M) and OTA (0.2 and 20 M) individually or collectively increased the paracellular flux of lucifer yellow and fluorescein isothiocyanate (FITC)-dextrans (4 and 40 kDa) and decreased transepithelial electrical resistance values in differentiated Caco-2 cells after 48 h of exposure, indicating increased epithelial permeability. Immunoblotting and immunofluorescent analysis revealed that AFM1, OTA, and their combination decreased the expression levels of tight junction (TJ) proteins and disrupted their structures, namely, claudin-3, claudin-4, occludin, and zonula occludens-1 (ZO-1), and p44/42 mitogen-activated protein kinase (MAPK) partially involved in the mycotoxins-induced disruption of intestinal barrier. The effects of a combination of AFM1 and OTA on intestinal barrier function were more significant (p < 0.05) than those of AFM1 and OTA alone, yielding additive or synergistic effects. The additive or synergistic effects of AFM1 and OTA on intestinal barrier function might affect human health, especially in children, and toxin risks should be considered.

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