Generation of mt:egfp transgenic zebrafish biosensor for the detection of aquatic zinc and cadmium

文献类型： 外文期刊

第一作者： Liu, Lili

作者： Liu, Lili;Yan, Yanchun;Wang, Jian;Wu, Wei;Xu, Lei

作者机构：

关键词： Transgenic zebrafish;Biomonitoring;Zinc;Cadmium;Water quality criteria

期刊名称：ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY （ 影响因子：3.742； 五年影响因子：3.866 ）

ISSN： 0730-7268

年卷期： 2016 年 35 卷 8 期

页码：

收录情况： SCI

摘要： Zebrafish embryo toxicity testing has become a popular method for detecting environmental pollutions. However, the present research showed that zebrafish embryos exhibited no visible paramorphia, malformation, or mortality when exposed to heavy metals in a range above environmental standard limits, indicating that zebrafish embryos are an imprecise model for monitoring environmental heavy metals concentrations above regulatory limits. Aiming to obtain a biosensor for aquatic heavy metals, a metal-sensitive vector including zebrafish metallothionein (MT) promoter and enhanced green fluorescent protein (EGFP) was reconstructed and microinjected into 1-cell stage zebrafish embryos. The authors obtained an mt:egfp transgenic zebrafish line sensitive to aquatic zinc and cadmium. A quantitative experiment showed that zinc and cadmium treatment significantly induced the expression of EGFP in a dose- and time-dependent manner. In particular, EGFP messenger RNA levels increased remarkably when exposed to heavy metals above the standard limits. The results suggest that the transgenic zebrafish is a highly sensitive biosensor for detecting environmental levels of zinc and cadmium. Environ Toxicol Chem 2016;35:2066-2073. (c) 2016 SETAC

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