Detection of p53 Protein Based on Mesoporous Pt-Pd Nanoparticles with Enhanced Peroxidase-like Catalysis

文献类型: 外文期刊

第一作者: Jiang, Tao

作者: Jiang, Tao;Liu, Xiangtao;Jiang, Tao;Song, Yang;Du, Dan;Lin, Yuehe;Lin, Yuehe;Du, Dan

作者机构:

关键词: immunochromatographic test strip;mesoporous Pt-Pd nanoparticles;peroxidase-like activity;TMB;H2O2

期刊名称:ACS SENSORS ( 影响因子:7.711; 五年影响因子:8.162 )

ISSN: 2379-3694

年卷期: 2016 年 1 卷 6 期

页码:

收录情况: SCI

摘要: We reported a sensitive immunochromato-graphic test strip based on mesoporous Pt-Pd bimetal nanoparticles (NPs) with greatly enhanced peroxidase-like catalysis for detection of p53 visually and quantitatively. The principle of this assay is relied on the measurements of visual color intensity produced by peroxidase reaction with 3,3',5,5'-tetramethylbenzidine (TMB) solution. Owing to the strong peroxidase-like activity of Pt-Pd NPs, it exhibited strong visual color in less than 30 s in low concentration range of target analytes. Quantitative measurement of p53 was performed with a hand-held test strip reader, which yields a detection limit of 0.05 ng mL(-1) with the linear range of 0.1-10 ng mL(-1.) Although the feasibility was demonstrated using p53 as a model analyte, this approach could be easily extended for detection of other protein biomarkers.

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