Dual Identification and Analysis of Differentially Expressed Transcripts of Porcine PK-15 Cells and Toxoplasma gondii during in vitro Infection

文献类型： 外文期刊

第一作者： Zhou, Chun-Xue

作者： Zhou, Chun-Xue;Suo, Xun;Zhou, Chun-Xue;Suo, Xun;Zhou, Chun-Xue;Zhou, Dong-Hui;Liu, Qing;Zhu, Xing-Quan;Elsheikha, Hany M.;Zhu, Xing-Quan

作者机构：

关键词： Toxoplasma gondii;PK-15 cells;host-pathogen interaction;transcriptome;KEGG;protein-protein interaction

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2016 年 7 卷

页码：

收录情况： SCI

摘要： Toxoplasma gondii is responsible for causing toxoplasmosis, one of the most prevalent zoonotic parasitoses worldwide. The mechanisms that mediate T. gondii infection of pigs (the most common source of human infection) and renal tissues are still unknown. To identify the critical alterations that take place in the transcriptome of both porcine kidney (PK-15) cells and T. gondii following infection, infected cell samples were collected at 1, 3, 6, 9, 12, 18, and 24 h post infection and RNA-Seq data were acquired using Illumina Deep Sequencing. Differential Expression of Genes (DEGs) analysis was performed to study the concomitant gene-specific temporal patterns of induction of mRNA expression of PK-15 cells and T gondii. High sequence coverage enabled us to thoroughly characterize T. gondii transcriptome and identify the activated molecular pathways in host cells. More than 6G clean bases/sample, including > 40 million clean reads were obtained. These were aligned to the reference genome of T. gondii and wild boar (Sus scrofa). DEGs involved in metabolic activities of T gondii showed time-dependent down-regulation. However, DEGs involved in immune or disease related pathways of PK-15 cells peaked at 6 h PI, and were highly enriched as evidenced by KEGG analysis. Protein-protein interaction analysis revealed that TGME49_120110 (PCNA), TGME49_049180 (DHFR-TS), TGME49_055320, and TGME49_002300 (lTPase) are the four hub genes with most interactions with T. gondii at the onset of infection. These results reveal altered profiles of gene expressed by PK-15 cells and T. gondii during infection and provide the groundwork for future virulence studies to uncover the mechanisms of T. gondii interaction with porcine renal tissue by functional analysis of these DEGs.

分类号：

  • 相关文献

[1]Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two-hybrid system. Liu, Qing,Zhu, Xing-Quan,Liu, Qing,Li, Fa-Cai,Sun, Miao-Miao,Zhu, Xing-Quan,Elsheikha, Hany M.,Zhu, Xing-Quan. 2017

[2]Toxoplasma gondii infection and schizophrenia: an inter-kingdom communication perspective. Elsheikha, Hany M.,Zhu, Xing-Quan.

[3]The known and missing links between Toxoplasma gondii and schizophrenia. Elsheikha, Hany M.,Busselberg, Dietrich,Zhu, Xing-Quan. 2016

[4]Transcriptomic analysis of mouse liver reveals a potential hepato-enteric pathogenic mechanism in acute Toxoplasma gondii infection. He, Jun-Jun,Ma, Jun,Song, Hui-Qun,Huang, Si-Yang,Zhu, Xing-Quan,Elsheikha, Hany M.,Ma, Jun,Huang, Si-Yang,Zhu, Xing-Quan. 2016

[5]Hepatic transcriptome analysis of juvenile GIFT tilapia (Oreochromis niloticus), fed diets supplemented with different concentrations of resveratrol. Zheng, Yao,Wu, Wei,Hu, Gengdong,Meng, Shunlong,Fan, Limin,Song, Chao,Qiu, Liping,Chen, Jiazhang,Zhao, Zhixiang,Chen, Jiazhang,Chen, Jiazhang. 2018

[6]Proteome analysis of egg yolk after exposure to zinc oxide nanoparticles. Zhang, Pengfei,Zhao, Yong,Yu, Shuai,Hao, Yanan,Ge, Wei,Min, Lingjing,Shen, Wei,Li, Lan,Liu, Jing,Zhang, Honfu,Li, Qunjie,Kou, Xin,Ma, Huanfa.

[7]Identification of salinity-related genes in ENO2 mutant (eno2(-)) of Arabidopsis thaliana. Zhang Yong-hua,Shi Zi-han,Cheng Hui-mei,Bing Jie,Ma Xiao-feng,Zheng Chao-xing,Zhang Gen-fa,Chen Chao,Li Hong-jie. 2018

[8]Nitric oxide-generating compound GSNO suppresses porcine circovirus type 2 infection in vitro and in vivo. Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang,Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang,Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang,Liu, Chuanmin,Wen, Libin,Xiao, Qi,He, Kongwang. 2017

[9]Inactivation of eGFP DNA in PK-15 cells via CRISPR/Cas9 system. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Liu, Maojun. 2017

[10]Complete nucleotide sequence of a novel porcine circo-virus-like agent and its infectivity in vitro. Wen LiBin,He KongWang,Ni YanXiu,Zhang XueHan,Guo RongLi,Pan QunXin,Yang HanChun. 2008

[11]A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses. Zheng Jingyuan,Mao Zhenchuan,Xie Bingyan,Zheng Jingyuan,Zou Xuexiao.

[12]Identification of a New Race of Sporisorium reilianum and Characterization of the Reaction of Sorghum Lines to Four Races of the Head Smut Pathogen. Zhang, Fuyao,Ping, Junai,Du, Zhihong,Cheng, Qingjun,Zhang, Fuyao,Huang, Yinghua,Zhang, Fuyao,Huang, Yinghua. 2011

[13]Floral transition in maize infected with Sporisorium reilianum disrupts compatibility with this biotrophic fungal pathogen. Zhang, Shaopeng,Xiao, Yannong,Zheng, Yonglian,Gardiner, Jack,Zhao, Jiuran,Wang, Fengge.

[14]De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica. Zhang, Xiao-Xuan,Ma, Jian-Gang,Zhao, Quan,Zhang, Xiao-Xuan,Cong, Wei,Ma, Jian-Gang,Zhu, Xing-Quan,Cong, Wei,Elsheikha, Hany M.,Liu, Guo-Hua,Huang, Wei-Yi,Zhu, Xing-Quan.

[15]Identification of defense-related genes in banana roots infected by Fusarium oxysporum f. sp cubense tropical race 4. Li, Weiming,Wang, Wei,Hu, Yulin,Mo, Yiwei,Sun, Dequan,Shi, Shengyou,Xie, Jianghui,Li, Weiming,Ge, Xuejun,Wu, Wei.

[16]Expression profiles of genes involved in TLRs and NLRs signaling pathways of water buffaloes infected with Fasciola gigantica. Zhang, Fu-Kai,Hou, Jun-Ling,Guo, Ai-Jiang,Tian, Ai-Ling,Zheng, Wen-Bin,Zhu, Xing-Quan,Sheng, Zhao-An,Huang, Wei-Yi,Elsheikha, Hany M.,Zhu, Xing-Quan. 2018

[17]Identification and functional analysis of the MOC1 interacting protein 1. Sun, Fengli,Zhang, Weiping,Xiong, Guosheng,Li, Jiayang,Wang, Yonghong,Sun, Fengli,Zhang, Weiping,Xiong, Guosheng,Li, Jiayang,Wang, Yonghong,Yan, Meixian,Qian, Qian. 2010

[18]A set of host proteins interacting with papaya ringspot virus NIa-Pro protein identified in a yeast two-hybrid system. Gao, L.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P.,Gao, L.,Shen, W. T.,Yan, P.,Tuo, D. C.,Li, X. Y.,Zhou, P.,Gao, L.. 2012

[19]Translation Initiation Factor eIF4E and eIFiso4E Are Both Required for Peanut stripe virus Infection in Peanut (Arachis hypogaea L.). Xu, Manlin,Xie, Hongfeng,Wu, Juxiang,Chi, Yucheng,Xu, Manlin,Xie, Lianhui,Yang, Jinguang. 2017

[20]Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system. Shen, W. T.,Wang, M. Q.,Yan, P.,Gao, L.,Zhou, P.. 2010

作者其他论文 更多>>

微信小程序